机构：[1]Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, People’s Republic of China[2]State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200032, People’s Republic of China[3]Institut Europeen des Membranes, UMR ENSCM-UM2-CNRS 5635, Universite Montpellier 2, Place Eugene Bataillon, F-34095 Montpellier Cedex 5, France
Biodegradable chitosan-graft-polylactide (PLA-CS) copolymers were prepared by the grafting of a poly(L-lactide) (PLLA) or poly(D-lactide) (PDLA) precursor to the backbone of chitosan using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC ⋅ HCl) and N-hydroxysuccinimide (NHS) as a coupling agent. The blood and cell compatibility of the graft copolymers were investigated in comparison to PLLA and PDLA homopolymers. The coagulation properties of PLA-CS were evaluated by hemolysis, plasma recalcification, dynamic blood clotting and protein absorption assays. PLA-CS copolymers present similar hemolysis ratio and plasma recalcification time as PLA, but slower dynamic blood clotting and lower protein absorption. The cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), agar diffusion and lactate dehydrogenase (LDH) experiments. All the samples presented no effect on the viability to cells. Inflammatory cytokine analysis using sandwich ELISAs revealed that PLA-CS would not stimulate inflammatory activity.
基金：
The work is supported by the 973 Program of Chinese
Government (no 2009C930300), the National Science
Foundation of People’s Republic of China (nos 30430770,
81272568 and 81101738) and the program for National
Samp;T Major project of China (2011ZX09501-001-01)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|2 区工程技术
小类|3 区工程：生物医学4 区材料科学：生物材料
最新[2023]版：
大类|3 区医学
小类|4 区工程：生物医学4 区材料科学：生物材料
第一作者：
第一作者机构：[1]Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, People’s Republic of China[2]State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200032, People’s Republic of China
通讯作者：
推荐引用方式(GB/T 7714)：
Qi Wang,Pei Liu,Peifeng Liu,et al.Preparation, blood coagulation and cell compatibility evaluation of chitosan-graft-polylactide copolymers.[J].Biomedical materials (Bristol, England).2014,9(1):015007.doi:10.1088/1748-6041/9/1/015007.
APA：
Qi Wang,Pei Liu,Peifeng Liu,Tao Gong,Suming Li...&Zhirong Zhang.(2014).Preparation, blood coagulation and cell compatibility evaluation of chitosan-graft-polylactide copolymers..Biomedical materials (Bristol, England),9,(1)
MLA：
Qi Wang,et al."Preparation, blood coagulation and cell compatibility evaluation of chitosan-graft-polylactide copolymers.".Biomedical materials (Bristol, England) 9..1(2014):015007
