机构：[1]College of Life Science, Jilin University, Changchun 130012, PR China[2]Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, PR China[3]Sichuan Tumor Hospital and Institute, Chengdu 610041, PR China四川省肿瘤医院
Nonviral gene delivery systems based on conventional high molecular weight chitosans are efficient as DNA vaccine delivery system, but have poor physical properties such as aggregated shapes, low solubility at neutral pH, high viscosity at concentrations used for in vivo delivery and a slow onset of action. Furthermore, Chitosan oligomers shorter than 14 monomers units were recently found to form only weak complexes with DNA, resulting in physically unstable polyplexes in vitro and in vivo. Here, low molecular weight chitosans with an average molecular mass of 6 kDa (Chito6) have been covalently attached to gold nanoparticles (GNPs), and the potency of the resulting Chito6-GNPs conjugates as vectors for the delivery of plasmid DNA has been investigated in vitro and in vivo. After delivery by intramuscular immunization in BALB/c mice, the Chito6-GNPs conjugates induced an enhanced serum antibody response 10 times more potent than naked DNA vaccine. Additionally, in contrast to naked DNA, the Chito6-GNPs conjugates induced potent cytotoxic T lymphocyte responses at a low dose. (c) 2007 Elsevier Ltd. All rights reserved.
基金：
Grant from
NSFC (20674029 and 30371317) to Wei Kong.
第一作者机构：[1]College of Life Science, Jilin University, Changchun 130012, PR China[2]Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, PR China
通讯作者：
通讯机构：[1]College of Life Science, Jilin University, Changchun 130012, PR China[2]Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, PR China[*1]College of Life Science, Jilin University, Changchun 130012, PR China.
推荐引用方式(GB/T 7714)：
Zhou Xianfeng,Zhang Xizhen,Yu Xianghul,et al.The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine[J].BIOMATERIALS.2008,29(1):111-117.doi:10.1016/j.biomaterials.2007.09.007.
APA：
Zhou, Xianfeng,Zhang, Xizhen,Yu, Xianghul,Zha, Xiao,Fu, Qiuan...&Shen, Jiacong.(2008).The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine.BIOMATERIALS,29,(1)
MLA：
Zhou, Xianfeng,et al."The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine".BIOMATERIALS 29..1(2008):111-117
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