机构：[1]National Engineering Research Center for Biomaterials, College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.[2]School of Materials and Chemistry, Southwest University of Science and Technology, Mianyang 621010, China.[3]School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China.[4]Department of Medical Oncology/Gastric Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.四川大学华西医院[5]Research Institute of Healthcare Science, Faculty of Science & Engineering, University of Wolverhampton, Wolverhampton WV1 1SB, UK.
It has been confirmed that skeletal muscle cells have the capability to receive foreign plasmid DNA (pDNA) and express functional proteins. This provides a promisingly applicable strategy for safe, convenient, and economical gene therapy. However, intramuscular pDNA delivery efficiency was not high enough for most therapeutic purposes. Some non-viral biomaterials, especially several amphiphilic triblock copolymers, have been shown to significantly improve intramuscular gene delivery efficiency, but the detailed process and mechanism are still not well understood. In this study, the molecular dynamics simulation method was applied to investigate the structure and energy changes of the material molecules, the cell membrane, and the DNA molecules at the atomic and molecular levels. From the results, the interaction process and mechanism of the material molecules with the cell membrane were revealed, and more importantly, the simulation results almost completely matched the previous experimental results. This study may help us design and optimize better intramuscular gene delivery materials for clinical applications.
基金：
This work was supported by the National Natural Science Foundation of China (31971390),
the 1.3.5 Project for Disciplines of Excellence,West China Hospital, Sichuan University (Grant No.
ZYJC21043), and the International Cooperative Project of Sichuan Province on Science and Technology
Innovation (No. 2021YFH0142).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|3 区医学
小类|3 区工程：生物医学3 区材料科学：生物材料
最新[2023]版：
大类|3 区医学
小类|3 区工程：生物医学3 区材料科学：生物材料
第一作者：
第一作者机构：[1]National Engineering Research Center for Biomaterials, College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Cui Zhanpeng,Jiao Yang,Pu Linyu,et al.The Interaction Mechanism of Intramuscular Gene Delivery Materials with Cell Membranes[J].Journal of functional biomaterials.2023,14(4):doi:10.3390/jfb14040219.
APA：
Cui Zhanpeng,Jiao Yang,Pu Linyu,Chen Jianlin,Liu Ming...&Wang Gang.(2023).The Interaction Mechanism of Intramuscular Gene Delivery Materials with Cell Membranes.Journal of functional biomaterials,14,(4)
MLA：
Cui Zhanpeng,et al."The Interaction Mechanism of Intramuscular Gene Delivery Materials with Cell Membranes".Journal of functional biomaterials 14..4(2023)
