Signal transducer and activator of transcription 3 (STAT3) is a member of the family of latent cytoplasmic transcriptional factors that could regulate cell proliferation, survival, and development. It has been reported that Twist is a target gene of STAT3, and STAT3/Twist signaling plays an important role in regulating cancer progress. Here, to explore whether 8-bromo-7-methoxychrysin (BrMC) inhibits liver cancer stem-like cell (LCSLC) properties via disrupting STAT3/Twist signaling, we cultured SMMC-7721 cells in vitro, and evaluated the effects of BrMC on the stemness of spheroids by determining the sphere-forming capability and migration. The sphere formation assay results showed a concentration-dependent decrease of sphere-forming capacity in LCSLCs (P < 0.05) treated with different concentrations of BrMC. Wound-healing assays results demonstrated a concentration-dependent decline in cell migration of LCSLCs treated with different concentrations of BrMC. In addition, CD133, CD44, and ALDH1 levels were decreased in LCSLCs treated with BrMC. Treatment with different concentrations of BrMC also reduced the expressions of p-STAT3 and Twist1 proteins. The effect of BrMC was substantially enhanced by co-treatment with JSI-124, a specific inhibitor of STAT3. Ectopic expression of Twist1 attenuated the inhibitory effects of BrMC on sphere formation, migration, and expression of the markers in LCSLCs. However, it had no affect on p-STAT3 expression in LCSLCs. These results demonstrated that BrMC inhibits the stemness of LCSLCs originated from SMMC-7721 cell line by inhibiting STAT3/Twist signal axis.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30760248, 81172375]; Project of Scientific Research of Hunan Province the Administration Bureau of Traditional Chinese Medicine [2010081]; Scientific Research of Hunan Province, Department of Education [10C0975]; Major Project Item of Scientific Research of Hunan Province the Department of Education [09A054]; Project of Scientific Research of Changsha City Bureau of Science and Technology [K1104060-31]; Construct Program of the Key Discipline in Hunan Province, China (Basic Medicine Sciences in University of South China)
语种：
外文
被引次数：
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中科院(CAS)分区：
出版当年[2017]版：
大类|3 区生物
小类|4 区生化与分子生物学4 区生物物理
最新[2023]版：
大类|2 区生物学
小类|2 区生物物理3 区生化与分子生物学
第一作者：
第一作者机构：[1]Univ South China, Coll Med, Inst Canc, Hengyang 421001, Peoples R China[2]Univ South China, Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Peoples R China[3]Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China
通讯作者：
通讯机构：[4]Hunan Normal Univ, Coll Med, Dept Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China
推荐引用方式(GB/T 7714)：
Luo Yimin,Cui Yinghong,Cao Xiaocheng,et al.8-Bromo-7-methoxychrysin-blocked STAT3/Twist axis inhibits the stemness of cancer stem cell-like cell originated from SMMC-7721 cells[J].ACTA BIOCHIMICA ET BIOPHYSICA SINICA.2017,49(5):458-464.doi:10.1093/abbs/gmx025.
APA：
Luo, Yimin,Cui, Yinghong,Cao, Xiaocheng,Li, Xiang,Chen, A....&Cao, Jianguo.(2017).8-Bromo-7-methoxychrysin-blocked STAT3/Twist axis inhibits the stemness of cancer stem cell-like cell originated from SMMC-7721 cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,49,(5)
MLA：
Luo, Yimin,et al."8-Bromo-7-methoxychrysin-blocked STAT3/Twist axis inhibits the stemness of cancer stem cell-like cell originated from SMMC-7721 cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 49..5(2017):458-464
