机构：[1]Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, the Second Clinical Medical College of North Sichuan Medical College, Nanchong, China
Testis expressed 10 (Tex10), a new core component of the pluripotency circuitry, has been reported to positively regulate embryonic stem cell (ESC) super-enhancers to promote ESC self-renewal; however, the expression and function of Tex10 in hepatocellular carcinoma (HCC) and liver cancer stem cells remains unclear. The present study was designed to investigate the expression patterns of Tex10 with immunohistochemistry, western blotting and RT-qPCR in samples from HCC patients and HCC cell lines. The results obtained show that Tex10 was highly expressed in HCC tissues, and elevated Tex10 protein levels positively correlate with the poorly differentiated carcinoma. Likewise, we found that Tex10 expression in the high-metastasis HCCLM3 potential cell line was higher than that in the low-metastasis HepG2 potential cell line, and Tex10 expression in liver cancer stem cells was also higher than that in adhered HCC cells. In addition, Tex10 knockdown decreased stem cell marker expression and drug resistance. Tex10 promoted cancer stemness through activation of the STAT3 signaling pathway. Taken together, our study demonstrates that Tex10 plays a potent carcinogenic role in HCC tumorigenesis by maintaining cancer stem cell properties through activation of the STAT3 signaling pathway and promoting chemo-resistance. Thus, targeting Tex10 may provide a novel and effective therapeutic strategy to suppress the tumorigenicity of advanced HCC.
基金：
This work was supported by the National Natural Science Fundation
of China (Grant No. 81201407), the Key Project of the Education
Department of Sichuan Province (Grant Nos. 16ZA0235, 17ZA0178),
the Innovation Team Project of Sichuan Provincial Education
Department (No. 13TD0030), the Major Transformation Cultivation
Project of Sichuan Provincial Education Department (No. 15CZ0021),
Bureau of Science & Technology and Intellectual Property Nanchong
City (NSMC20170466, 16YFZJ0031), the Sichuan Provincial Health
and Family Planning Commission research project (No. 17PJ487,
No. 17PJ503), and the Applied Fundamental Program of Science and
Technology Department of Sichuan Province (No. 2016JY0123).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|2 区生物
小类|3 区细胞生物学
最新[2023]版：
大类|3 区生物学
小类|4 区细胞生物学
第一作者：
第一作者机构：[1]Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, the Second Clinical Medical College of North Sichuan Medical College, Nanchong, China
通讯作者：
推荐引用方式(GB/T 7714)：
Xiaocong Xiang,Li Deng,Rong Xiong,et al.Tex10 is upregulated and promotes cancer stem cell properties and chemoresistance in hepatocellular carcinoma.[J].Cell cycle (Georgetown, Tex.).2018,17(11):1310-1318.doi:10.1080/15384101.2018.1480208.
APA：
Xiaocong Xiang,Li Deng,Rong Xiong,Dongqin Xiao,Zhu Chen...&Gang Feng.(2018).Tex10 is upregulated and promotes cancer stem cell properties and chemoresistance in hepatocellular carcinoma..Cell cycle (Georgetown, Tex.),17,(11)
MLA：
Xiaocong Xiang,et al."Tex10 is upregulated and promotes cancer stem cell properties and chemoresistance in hepatocellular carcinoma.".Cell cycle (Georgetown, Tex.) 17..11(2018):1310-1318
