机构：[1]Department of Breast Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu 611731,China四川省人民医院[2]Department of Breast Surgery, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 611731, China外科中心乳腺外科中心四川省人民医院四川省肿瘤医院乳腺科[3]Department of Breast and Thyroid Surgery, The Third Affiliated Hospital of Zunyi Medical University (The First People’s Hospital of Zunyi), Zunyi 563000, China
Background: CD44(+)/CD24(-/low) and ALDH1+ are both markers of breast cancer stem cell (BCSC), we compared the biological characteristics of CD44(+)/CD24(-/low) and ALDH1+ BCSCs in the four molecular subtypes of breast cancer. Methods: Four fresh blocks of breast cancer tissue were obtained from patients with Luminal A, Luminal B, human epidermal receptor-2 (HER2)-overexpression and triple-negative breast cancer, respectively. Flow cytometry was used to sort CD44(+)/CD24(-/low) and ALDH1+ BCSCs. The mammosphere (MS) formation experiment and NOD/SCID mouse xenograft experiment were performed to compare the self-renewal and tumorigenic abilities of CD44(+)/CD24(-/low) with those of ALDH1+ BCSCs. Results: The proportions of CD44(+)/CD24(-/low) BCSC and ALDH1+ BCSC in Luminal A, Luminal B, HER2 overexpression and triple-negative subtypes were 12.1% and 8.7%, 2.7% and 5.7%, 0.8% and 8.7%, 0.7% and 4.5%, respectively. The MS formation experiment demonstrated that ALDH1+ BCSC formed significantly more MSs than CD44(+)/CD24(-/low) BCSC in the four molecular subtypes (P<0.001). The NOD/SCID mouse xenograft experiment demonstrated that the sizes of grafted tumors formed by ALDH1+ BCSC were larger than those formed by CD44(+)/CD24(-/low) BCSC in the four molecular subtypes (P<0.05). Conclusions: In the four molecular subtypes of breast cancer, ALDH1+ BCSC demonstrated better selfrenewal and tumorigenic abilities than CD44(+)/CD24(-/low) BCSC.
基金：
Doctor's Fund of Sichuan Provincial People's Hospital
第一作者机构：[1]Department of Breast Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu 611731,China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Breast Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu 611731,China[3]Department of Breast and Thyroid Surgery, The Third Affiliated Hospital of Zunyi Medical University (The First People’s Hospital of Zunyi), Zunyi 563000, China[*1]Department of Breast Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu 611731, China[*2]Department of Breast and Thyroid Surgery, The Third Affiliated Hospital of Zunyi Medical University (The First People’s Hospital of Zunyi), Zunyi 563000, China
推荐引用方式(GB/T 7714)：
Chen Jie,Liu Shiwei,Su Yingying,et al.ALDH1+stem cells demonstrate more stem cell-like characteristics than CD44(+)/CD24(-/low) stem cells in different molecular subtypes of breast cancer[J].TRANSLATIONAL CANCER RESEARCH.2020,9(3):1652-1659.doi:10.21037/tcr.2020.01.53.
APA：
Chen, Jie,Liu, Shiwei,Su, Yingying&Zhang, Xiao.(2020).ALDH1+stem cells demonstrate more stem cell-like characteristics than CD44(+)/CD24(-/low) stem cells in different molecular subtypes of breast cancer.TRANSLATIONAL CANCER RESEARCH,9,(3)
MLA：
Chen, Jie,et al."ALDH1+stem cells demonstrate more stem cell-like characteristics than CD44(+)/CD24(-/low) stem cells in different molecular subtypes of breast cancer".TRANSLATIONAL CANCER RESEARCH 9..3(2020):1652-1659
