机构：[1]West China Hospital Laboratory of Nanomedicine and Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu, China[2]Key Laboratory of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu, China四川大学华西医院[3]Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
A nanoscale injectable in situ-forming hydrogel drug delivery system was developed in this study. The system was based on a self-assembling peptide RADA16 solution, which can spontaneously form a hydrogel rapidly under physiological conditions. We used the RADA16 hydrogel for the controlled release of paclitaxel (PTX), a hydrophobic antitumor drug.
The RADA16-PTX suspension was prepared simply by magnetic stirring, followed by atomic force microscopy, circular dichroism analysis, dynamic light scattering, rheological analysis, an in vitro release assay, and a cell viability test.
The results indicated that RADA16 and PTX can interact with each other and that the amphiphilic peptide was able to stabilize hydrophobic drugs in aqueous solution. The particle size of PTX was markedly decreased in the RADA16 solution compared with its size in water. The RADA16-PTX suspension could form a hydrogel in culture medium, and the elasticity of the hydrogel showed a positive correlation with peptide concentration. In vitro release measurements indicated that hydrogels with a higher peptide concentration had a longer half-release time. The RADA16-PTX hydrogel could effectively inhibit the growth of the breast cancer cell line, MDA-MB-435S, in vitro, and hydrogels with higher peptide concentrations were more effective at inhibiting tumor cell proliferation. The RADA16-PTX hydrogel was effective at controlling the release of PTX and inhibiting tumor cell growth in vitro.
Self-assembling peptide hydrogels may work well as a system for drug delivery.
基金：
This work was supported
by the Chinese National 985 Project of Education
Ministry to Sichuan University and by the National Natural
Science Foundation of china (Grant No. 31070889).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2011]版：
大类|3 区医学
小类|3 区纳米科技3 区药学
最新[2023]版：
大类|2 区医学
小类|2 区药学3 区纳米科技
第一作者：
第一作者机构：[1]West China Hospital Laboratory of Nanomedicine and Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu, China[2]Key Laboratory of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu, China
通讯作者：
通讯机构：[1]West China Hospital Laboratory of Nanomedicine and Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu, China[3]Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA[*1]West China Hospital Laboratory of Nanomedicine and Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, 1 Ke Yuan 4th Street, 610041, Chengdu, China
推荐引用方式(GB/T 7714)：
Jingping Liu,Lanlan Zhang,Zehong Yang,et al.Controlled release of paclitaxel from a self-assembling peptide hydrogel formed in situ and antitumor study in vitro.[J].International journal of nanomedicine.2011,6:2143-53.doi:10.2147/IJN.S24038.
APA：
Jingping Liu,Lanlan Zhang,Zehong Yang&Xiaojun Zhao.(2011).Controlled release of paclitaxel from a self-assembling peptide hydrogel formed in situ and antitumor study in vitro..International journal of nanomedicine,6,
MLA：
Jingping Liu,et al."Controlled release of paclitaxel from a self-assembling peptide hydrogel formed in situ and antitumor study in vitro.".International journal of nanomedicine 6.(2011):2143-53
医学