A Miniature Cell Pattern Formation of Ovarian Cancer Cell Lines on Self-Assembling Peptide Nanofiber-Coated Coverslip and In Vitro Chemosensitivity Assay.
机构：[1]Biomolecular Medicine Lab, West China College of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, P. R. China.[2]West China Hospital Nanomedicine Laboratory, Institute for Nanobiomedical Technology and Membrane Biology, Department of Ultrasound, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, P. R. China.四川大学华西医院[3]Department of Microbiology, College of Basic Medicine, Zunyi Medical University, Huichuan District, Zunyi 563003, Guizhou, P. R. China.[4]Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, Sichuan, P. R. China.
Despite of intensive efforts, the tumor progression of ovarian cancer has not yet been fully understood. Various studies suggest that an effective experimental model system In Vitro to mimic microenvironment in vivo and the fabrication of cell pattern formation In Vitro are of great importance for the ovarian cancer research.
This study cultivates SKOV3, A2780 and A2780/DDP ovarian cancer cell lines in RADA16-I peptide hydrogel to obtain 3D-precultured cancer cells and fabricate a type of miniature cell pattern formation In Vitro on RADA16-I peptide nanofibercoated coverslip by in situ AFM analysis and chemosensitivity assay in vitro.
SKOV3, A2780 and A2780/DDP cells were regularly cultivated in RADA16-I peptide hydrogel, respectively, and present defined cellular characteristics, such as small cell colonies, cell clusters and cell aggregates, etc. On coverslip coated with RADA16-I peptide nanofiber, three 3D-precultured cell lines were flat and spread on coverslips and the regular topographic images by AFM were obtained. The chemosensitivity assay of three ovarian cancer cell lines were significantly different in RADA16-I peptide-coated coverslips compared with 2D dish plates.
By 3D cell culture of ovarian cancer cell lines in RADA16-I peptide hydrogel In Vitro, the cell culture model systems was fabricated. The miniature cell pattern formation In Vitro on peptide nanofiber-coated coverslip was particularly useful experiemental model systems to be served as a valuable methodology for studying ovarian cancer biology.
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出版当年[2018]版：
大类|4 区工程技术
小类|4 区化学综合4 区材料科学：综合4 区纳米科技4 区物理：应用4 区物理：凝聚态物理
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第一作者：
第一作者机构：[1]Biomolecular Medicine Lab, West China College of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, P. R. China.[2]West China Hospital Nanomedicine Laboratory, Institute for Nanobiomedical Technology and Membrane Biology, Department of Ultrasound, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, P. R. China.
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推荐引用方式(GB/T 7714)：
Yang Zehong,Zhuang Hua,Song Hong,et al.A Miniature Cell Pattern Formation of Ovarian Cancer Cell Lines on Self-Assembling Peptide Nanofiber-Coated Coverslip and In Vitro Chemosensitivity Assay.[J].Journal of nanoscience and nanotechnology.2018,18(4):2370-2378.doi:10.1166/jnn.2018.14384.
APA：
Yang Zehong,Zhuang Hua,Song Hong,Liu Ji,Zhao Xiaojun&Lin Wei.(2018).A Miniature Cell Pattern Formation of Ovarian Cancer Cell Lines on Self-Assembling Peptide Nanofiber-Coated Coverslip and In Vitro Chemosensitivity Assay..Journal of nanoscience and nanotechnology,18,(4)
MLA：
Yang Zehong,et al."A Miniature Cell Pattern Formation of Ovarian Cancer Cell Lines on Self-Assembling Peptide Nanofiber-Coated Coverslip and In Vitro Chemosensitivity Assay.".Journal of nanoscience and nanotechnology 18..4(2018):2370-2378
