机构：[1]Molecular Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.[2]State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.[3]Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.[4]Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Graduate School of Dentistry, Tohoku University, Sendai, Japan.[5]Lifecare Acupuncture and Alternative Medicine Center, Colleyville, TX, USA.[6]Department of Pathophysiology for Locomotive and Neoplastic Diseases, Graduate School of Medicine, Juntendo University, Tokyo, Japan.[7]Department of Orthopedic Surgery, Oita University, Oita, Japan.[8]Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.[9]Department of Medicine for Orthopedics and Motor Organ, Graduate School of Medicine, Juntendo University, Tokyo, Japan.[10]Pediatric Dentistry, St. Mary's Hospital, Kurume, Japan.[11]Division of Operative Dentistry, Laboratory of Cell and Department of Restorative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan.[12]Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Nagoya University, Nagoya, Japan.
Basic-helix-loop-helix (bHLH) transcription factors play an important role in various organs' development; however, a tooth-specific bHLH factor has not been reported. In this study, we identified a novel tooth-specific bHLH transcription factor, which we named AmeloD, by screening a tooth germ complementary DNA (cDNA) library using a yeast 2-hybrid system. AmeloD was mapped onto the mouse chromosome 1q32. Phylogenetic analysis showed that AmeloD belongs to the achaete-scute complex-like ( ASCL) gene family and is a homologue of ASCL5. AmeloD was uniquely expressed in the inner enamel epithelium (IEE), but its expression was suppressed after IEE cell differentiation into ameloblasts. Furthermore, AmeloD expression showed an inverse expression pattern with the epithelial cell-specific cell-cell adhesion molecule E-cadherin in the dental epithelium. Overexpression of AmeloD in dental epithelial cell line CLDE cells resulted in E-cadherin suppression. We found that AmeloD bound to E-box cis-regulatory elements in the proximal promoter region of the E-cadherin gene. These results reveal that AmeloD functions as a suppressor of E-cadherin transcription in IEE cells. Our study demonstrated that AmeloD is a novel tooth-specific bHLH transcription factor that may regulate tooth development through the suppression of E-cadherin in IEE cells.
基金：
Intramural Research
Program of the National Institute of Dental and Craniofacial
Research, National Institutes of Health (Y. Yamada). This work
was also supported by the National Natural Science Foundation of
China (NSFC81500811 to B. He) and by the Japan Society for the
Promotion of Science KAKENHI Grant-in-Aid (15J04116 to Y.
Chiba).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|1 区医学
小类|1 区牙科与口腔外科
最新[2023]版：
大类|1 区医学
小类|1 区牙科与口腔外科
第一作者：
第一作者机构：[1]Molecular Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.[2]State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.[3]Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
通讯作者：
通讯机构：[1]Molecular Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.[*1]Molecular Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive MSC 4370, Bethesda, MD 20892-4370, USA.
推荐引用方式(GB/T 7714)：
He B,Chiba Y,Li H,et al.Identification of the Novel Tooth-Specific Transcription Factor AmeloD.[J].Journal of dental research.2019,98(2):234-241.doi:10.1177/0022034518808254.
APA：
He B,Chiba Y,Li H,de Vega S,Tanaka K...&Yamada Y.(2019).Identification of the Novel Tooth-Specific Transcription Factor AmeloD..Journal of dental research,98,(2)
MLA：
He B,et al."Identification of the Novel Tooth-Specific Transcription Factor AmeloD.".Journal of dental research 98..2(2019):234-241
医学