机构：[1]West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, Sichuan, China[2]Department of clinical nutrition, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610042, Sichuan, China四川省人民医院四川省肿瘤医院[3]Chengdu Institute of Product Quality Inspection Co., Ltd, Chengdu 610015, China
Okra flower is an untapped flavonoid-rich resource, and flavonoids from it (AFE) have been demonstrated intestinal protective potential due to anti-colorectal cancer properties, but other intestinal-related effects remain to be explored. Here, we found that AFE, as a safe natural product, effectively alleviate 2.5% dextran sulfate sodium (DSS)-induced intestinal inflammation in C57BL/6N and DSS-induced injury of human intestinal epithelial cells NCM460. AFE maintained colon integrity by promoting the levels of tight junction proteins, ZO-1 and Occludin, and modulating the NF-kappa B signaling pathway to prevent further tissue damage. Meanwhile, gut microbiota was involved in the protective effect of AFE in vivo, with Akkermansia making a major contribution. Moreover, AFE exhibited similar inhibitory effects on the NF-kappa B pathway in vitro and exerted antioxidant effects as a ROS scavenger by regulating the Nrf2 signaling pathway. In summary, this study demonstrated the potential of AFE in restoring intestinal health in vitro and in vivo.
基金：
This research was funded by Sichuan University, Grant No 19H0749
第一作者机构：[1]West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, Sichuan, China
通讯作者：
通讯机构：[1]West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, Sichuan, China[*1]West China School of Public Health and West China Fourth Hospital, Sichuan University, 17# 3rd Section, Ren Min South Road, 610041 Chengdu, China
推荐引用方式(GB/T 7714)：
Huang Xiaoyi,Li Xingjie,Deng Yuanle,et al.The flavonoids extract from Okra flowers protects against DSS-induced colitis via regulating NF-kappa B signaling pathway and gut microbiota[J].JOURNAL OF FUNCTIONAL FOODS.2022,99:doi:10.1016/j.jff.2022.105335.
APA：
Huang, Xiaoyi,Li, Xingjie,Deng, Yuanle,Zhou, Ting,Chen, Tao...&Yin, Wenya.(2022).The flavonoids extract from Okra flowers protects against DSS-induced colitis via regulating NF-kappa B signaling pathway and gut microbiota.JOURNAL OF FUNCTIONAL FOODS,99,
MLA：
Huang, Xiaoyi,et al."The flavonoids extract from Okra flowers protects against DSS-induced colitis via regulating NF-kappa B signaling pathway and gut microbiota".JOURNAL OF FUNCTIONAL FOODS 99.(2022)
农林科学