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Characterization of the microRNA transcriptomes and proteomics of cochlear tissue-derived small extracellular vesicles from mice of different ages after birth.

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机构： [1]State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Jiangsu Province High‑Tech Key Laboratory for Bio‑Medical Research, Southeast University, Nanjing 210096, China [2]State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China [3]Department of Chemistry, Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA [4]Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA [5]Co‑Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China [6]Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China [7]Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing, China [8]Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China

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关键词： Small extracellular vesicles Cochlea Development MicroRNAs Proteins Proteomics

摘要：

The cochlea is an important sensory organ for both balance and sound perception, and the formation of the cochlea is a complex developmental process. The development of the mouse cochlea begins on embryonic day (E)9 and continues until postnatal day (P)21 when the hearing system is considered mature. Small extracellular vesicles (sEVs), with a diameter ranging from 30 to 200 nm, have been considered a significant medium for information communication in both physiological and pathological processes. However, there are no studies exploring the role of sEVs in the development of the cochlea. Here, we isolated tissue-derived sEVs from the cochleae of FVB mice at P3, P7, P14, and P21 by ultracentrifugation. These sEVs were first characterized by transmission electron microscopy, nanoparticle tracking analysis, and western blotting. Next, we used small RNA-seq and mass spectrometry to characterize the microRNA transcriptomes and proteomes of cochlear sEVs from mice at different ages. Many microRNAs and proteins were discovered to be related to inner ear development, anatomical structure development, and auditory nervous system development. These results all suggest that sEVs exist in the cochlea and are likely to be essential for the normal development of the auditory system. Our findings provide many sEV microRNA and protein targets for future studies of the roles of cochlear sEVs.© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.

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出版当年[2022]版：

大类 | 1 区

生物学

小类 | 2 区细胞生物学 2 区生化与分子生物学

最新[2025]版：

大类 | 2 区

生物学

小类 | 2 区生化与分子生物学 2 区细胞生物学

JCR分区：

出版当年[2022]版：

Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Q1 CELL BIOLOGY

最新[2024]版：

Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Q1 CELL BIOLOGY

影响因子： 6.2 最新[2024版] 7.7 最新五年平均 8 出版当年[2022版] 8.7 出版当年五年平均 9.234 出版前一年[2021版] 6.2 出版后一年[2023版]

第一作者：

第一作者机构： [1]State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Jiangsu Province High‑Tech Key Laboratory for Bio‑Medical Research, Southeast University, Nanjing 210096, China

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通讯作者：

通讯机构： [1]State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Jiangsu Province High‑Tech Key Laboratory for Bio‑Medical Research, Southeast University, Nanjing 210096, China [3]Department of Chemistry, Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA [4]Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA [5]Co‑Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China [6]Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China [7]Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing, China [8]Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China

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Characterization of the microRNA transcriptomes and proteomics of cochlear tissue-derived small extracellular vesicles from mice of different ages after birth.

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