机构：[1]School of medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China四川省人民医院[2]Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan, Cancer Center, School of Medicine University, Sichuan, China四川省肿瘤医院[3]The General Hospital of Western Theater Command, Chengdu, Sichuan, China[4]Guangxi Medical University, Nanning, Guangxi, P.R. China
MiR-326 functions as an antioncogene in the several types of cancer. However, the underling mechanisms through which miRNA-326 regulates the anti-carcinogenesis of lung adenocarcinoma have remained elusive. The aim of this study was to explore the role and regulatory mechanism of miR-326 in cell proliferation, invasion, migration and apoptosis in lung adenocarcinoma. Quantitative real-time PCR (qRT-PCR) was used to detect the expression pattern of miR-326 in human bronchial epithelial cells (HBES-2B), 4 kinds of lung adenocarcinoma cell lines (H23, H1975, H2228, H2085) and 20 lung adenocarcinoma tissues. Then, H23 cells were infected with miR-326 mimics, miR-326 inhibitors and si-ZEB1 to build up-regulated miR-326 cell lines, down-regulated ZEB1(zinc-finger-enhancer binding protein 1)cell lines, simultaneous down-regulated ZEB1 and miR-326 cell lines. Moreover, CCK-8 assay, transwell invasion assay, wound healing assay and flow cytometry assay were employed to examine the effects of miR-326 and ZEB1 on the proliferation, invasion, migration and apoptosis abilities of H23 cells. Western blot was performed to explore the effects of miR-326 and ZEB1 on the expression of invasion and migration related proteins N-cadherin, E-cadherin, MMP7, MMP13, SLUG and apoptotic proteins PARP, BAX. On the mechanism, a dual-luciferase reporter gene was used to measure the target relationship between miR-326 and ZEB1. MiR-326 expression was significantly downregulated in lung adenocarcinoma tissues and cells. Overexpression of miR-326 significantly inhibited the malignant behaviors of H23 cells. Mechanically, luciferase reporter assay showed that ZEB1 was a direct target of miR-326. MiR-326 mimic downregulated the expression of ZEB1. Furthermore, knocking down ZEB1 strongly inhibited the proliferation, invasion and migration of H23 cells but promoted apoptosis. MiR-326 could target ZEB1 to inhibit the proliferation, invasion and migration of lung adenocarcinoma cells and promote apoptosis, which is a potential therapeutic target for lung adenocarcinoma.
基金：
The author(s) disclosed receipt of the following financial support for the research, authorship
and/or publication of this article: The present study was supported by the National Natural
Science Foundation of China (No. 81873048) and Sichuan science and technology department key research and development projects (2017SZ0013).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|4 区综合性期刊
小类|4 区综合性期刊
最新[2023]版：
大类|4 区综合性期刊
小类|4 区综合性期刊
第一作者：
第一作者机构：[1]School of medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China[2]Integrative Cancer Center & Cancer Clinical Research Center, Sichuan Cancer Hospital & Institute Sichuan, Cancer Center, School of Medicine University, Sichuan, China
共同第一作者：
通讯作者：
通讯机构：[1]School of medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China[*1]School of Medicine, University of Electronic Science and Technology of China, No. 4, Section 2, Jianshe North Road, Chenghua District, Chengdu, Sichuan 610000, P.R. China
推荐引用方式(GB/T 7714)：
Liu Mingxin,Wu Hong,Liu Yiqiang,et al.MiR-326 mediates malignant biological behaviors of lung adenocarcinoma by targeting ZEB1.[J].Science progress.2021,104(2):368504211009379.doi:10.1177/00368504211009379.
APA：
Liu Mingxin,Wu Hong,Liu Yiqiang,Tan Yan,Wang Songtao...&Li Qiang.(2021).MiR-326 mediates malignant biological behaviors of lung adenocarcinoma by targeting ZEB1..Science progress,104,(2)
MLA：
Liu Mingxin,et al."MiR-326 mediates malignant biological behaviors of lung adenocarcinoma by targeting ZEB1.".Science progress 104..2(2021):368504211009379
