机构：[1]Department of Liver Surgery, Liver Transplantation Division, West China Hospital, Sichuan University, Chengdu, China四川大学华西医院[2]Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China四川大学华西医院[3]Department of Bioengineering and Therapeutic Sciences and Liver Center, University of California, San Francisco, California[4]Institute of Pathology, University of Regensburg, Regensburg, Germany
Liver cancer is the second most lethal malignancy worldwide. Cell lines and murine models are the most common tools for modeling human liver carcinogenesis. Most recently, organoids with a three-dimensional structure derived from primary tissues or cells have been applied to liver cancer research. Organoids can be generated from induced pluripotent stem cells, embryonic or adult, healthy or diseased tissues. In particular, liver organoids have been widely employed in mechanistic studies aimed at delineating the molecular pathways responsible for hepatocarcinogenesis. The introduction of clustered regularly interspaced palindromic repeats (CRISPR)-associated protein 9 (Cas9) and microengineered miniorganoid technologies into liver organoids for cancer study has significantly accelerated these investigations. Translational advances have been made by utilizing liver tumor organoids for anticancer drug screening, biobanking, omics profiling, and biomarker discovery. This review summarizes the latest advances and the remaining challenges in the use of organoid models for the study of liver cancer.
Thieme. All rights reserved.
基金：
This study is supported by grants from the National Institute of Health (P30DK026743, R01CA190606, R01CA239251) and West China Hospital, Sichuan University Post-Doctoral Research Project (2020HXBH006)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|2 区医学
小类|2 区胃肠肝病学
最新[2023]版：
大类|3 区医学
小类|4 区胃肠肝病学
第一作者：
第一作者机构：[1]Department of Liver Surgery, Liver Transplantation Division, West China Hospital, Sichuan University, Chengdu, China[2]Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China[3]Department of Bioengineering and Therapeutic Sciences and Liver Center, University of California, San Francisco, California
通讯作者：
通讯机构：[3]Department of Bioengineering and Therapeutic Sciences and Liver Center, University of California, San Francisco, California[*1]513 Parnassus Ave., University of California San Francisco, CA 94143
推荐引用方式(GB/T 7714)：
Wang Haichuan,Calvisi Diego F,Chen Xin.Organoids for the Study of Liver Cancer.[J].Seminars in liver disease.2021,41(1):19-27.doi:10.1055/s-0040-1719176.
APA：
Wang Haichuan,Calvisi Diego F&Chen Xin.(2021).Organoids for the Study of Liver Cancer..Seminars in liver disease,41,(1)
MLA：
Wang Haichuan,et al."Organoids for the Study of Liver Cancer.".Seminars in liver disease 41..1(2021):19-27
：