机构：[1]Department of Paediatrics, Western Women’s and Children’s Research Institute, West China University Second Hospital, Sichuan University, Number 20, 3rd Section, People’s South Road, Chengdu 610041, Sichuan Province, China.[2]Key Laboratory of Birth Defects and Related Diseases of Women and Children, (Sichuan University), Ministry of Education, Chengdu 610041, Sichuan, China.[3]Open Laboratory, West China Institute for Women’s and Children’s Health, Chengdu 610041, Sichuan, China.[4]Group of bacterial biology, Department of Laboratory Medicine, Sichuan university west China second hospital, Chengdu 610041, Sichuan, China.
Previous studies have shown that changes in intestinal microfloras are associated with both gastrointestinal (GI) and non-GI tumors. It is not clear whether there is an association between GI microflora changes and hematological malignancies.
In the current study, we used 16S rDNA gene sequencing techniques to profile the GI microbiome in children with lymphoblastic leukemia (ALL, n = 18) and matched healthy control (n = 18). Using multiple specialized software [Heatmap, Principal coordinates analysis (PCoA), Claster and Metastates], we analyzed the sequencing data for microfloral species classification, abundance and diversity.
A total of 27 genera between the ALL and control groups (FDR ≤ 0.05 and/or P ≤ 0.05) showed significantly different abundance between ALL patients and healthy controls: 12 of them were predominant in healthy group and other 15 species were significantly higher in ALL group. In addition, we compared the abundance and diversity of microfloral species in ALL patients prior to and during remission stage after chemotherapy, and no significant difference was detected.
Compared to healthy controls, ALL patient showed significant changes of GI microfloras. Further explorations of the intestinal micro-ecology in ALL patients may provide important information to understand relationship between microfloras and ALL.
基金：
The authors would like to thank all study subjects and the medical staff
involved in this study. All phases of this study were supported by grants
from the Sichuan Provincial Health Commission Research Topic
(NO.19PJYY0042), the Huimin Project of the Chengdu City Science and
Technology Bureau (NO.2014-HM01-00017-SF), and the Sichuan University,
West China Second Hospital Clinical Scientific Research Project Funds
(NO.2016-KL010).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区医学
小类|3 区儿科
最新[2023]版：
大类|3 区医学
小类|3 区儿科
第一作者：
第一作者机构：[1]Department of Paediatrics, Western Women’s and Children’s Research Institute, West China University Second Hospital, Sichuan University, Number 20, 3rd Section, People’s South Road, Chengdu 610041, Sichuan Province, China.[2]Key Laboratory of Birth Defects and Related Diseases of Women and Children, (Sichuan University), Ministry of Education, Chengdu 610041, Sichuan, China.
通讯作者：
通讯机构：[1]Department of Paediatrics, Western Women’s and Children’s Research Institute, West China University Second Hospital, Sichuan University, Number 20, 3rd Section, People’s South Road, Chengdu 610041, Sichuan Province, China.[2]Key Laboratory of Birth Defects and Related Diseases of Women and Children, (Sichuan University), Ministry of Education, Chengdu 610041, Sichuan, China.
推荐引用方式(GB/T 7714)：
Xiaolin Gao,Ruixue Miao,Yiping Zhu,et al.A new insight into acute lymphoblastic leukemia in children: influences of changed intestinal microfloras.[J].BMC pediatrics.2020,20(1):290.doi:10.1186/s12887-020-02192-9.
APA：
Xiaolin Gao,Ruixue Miao,Yiping Zhu,Chao Lin,Xue Yang...&Jianjun Deng.(2020).A new insight into acute lymphoblastic leukemia in children: influences of changed intestinal microfloras..BMC pediatrics,20,(1)
MLA：
Xiaolin Gao,et al."A new insight into acute lymphoblastic leukemia in children: influences of changed intestinal microfloras.".BMC pediatrics 20..1(2020):290
