机构：[1]Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, 610041 Chengdu, Sichuan, China四川大学华西医院[2]Center for Biomedical Engineering, NE47-379, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA
The anticancer properties and mechanism of action of a hybrid peptide -P18 were investigated. It had significant cytotoxic activity against human melanoma cells and low toxicity to normal NIH-3T3 cells. It also induced cell death via necrosis rather than classical apoptosis. The peptide targets the cells membrane, causing a sustained depolarization of transmembrane potential resulting in the cells swelling and bursting, thereby triggering cytolysis. P18 peptide initially binds to the melanoma cell membrane via electrostatic interaction, causing the cell membrane to rupture. The effect may be mediated by the amphiphilic alpha-helical structure of P18 peptide, coupled with changes in ion channels and an increase in plasma membrane permeability that eventually leads to melanoma cell death.
基金：
National 985 Key Project of Sichuan University of the
Education Ministry of China
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2010]版：
大类|3 区工程技术
小类|4 区生物工程与应用微生物
最新[2023]版：
大类|4 区生物学
小类|4 区生物工程与应用微生物
第一作者：
第一作者机构：[1]Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, 610041 Chengdu, Sichuan, China
通讯作者：
通讯机构：[1]Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, 610041 Chengdu, Sichuan, China[2]Center for Biomedical Engineering, NE47-379, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA
推荐引用方式(GB/T 7714)：
Huang Wenli,Lu Li,Shao Ximing,et al.Anti-melanoma activity of hybrid peptide P18 and its mechanism of action.[J].Biotechnology letters.2010,32(4):463-9.doi:10.1007/s10529-009-0175-2.
APA：
Huang Wenli,Lu Li,Shao Ximing,Tang Chengkang&Zhao Xiaojun.(2010).Anti-melanoma activity of hybrid peptide P18 and its mechanism of action..Biotechnology letters,32,(4)
MLA：
Huang Wenli,et al."Anti-melanoma activity of hybrid peptide P18 and its mechanism of action.".Biotechnology letters 32..4(2010):463-9
