机构：[1]Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education , Chengdu, Sichuan 610041 , P.R. China[2]Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children ’ s Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041 , P.R. China[3]Department of Forensic Biology , West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041 , P.R. China[4]Department of Forensic Pathology , Henan University of Science and Technology, Luoyang, Henan 471003 , P.R. China[5]Secondary Department of General Surgery , Luo Yang Central Hospital, Luoyang, Henan 471003 , P.R. China[6]PLA Institute of Physical Education, Guangzhou, Guangdong 510502 , P.R. China
The miR-34 family members, described as potential tumor suppressors, were downregulated in colorectal cancer (CRC). Loss of miR-34 impairs TP53-mediated cell death, while overexpression of miR-34 induces apoptosis. A potentially functional polymorphism (i.e., rs4938723T/C) in the promoter region of pri-miR-34b/c was predicted to influence the GATA-X binding sites. We aimed to investigate the association between miR-34b/c rs4938723 and TP53 Arg72Pro polymorphisms and the risk of CRC. We genotyped the two polymorphisms in 347 CRC patients and 488 healthy controls using polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing assay. We found that the CC genotype and C allele of the miR-34b/c rs4938723 were associated with a significantly decreased risk of CRC compared with the TT genotype and T allele (CC vs. TT: adjusted OR=0.56; 95% CI, 0.34-0.91; C vs. T: adjusted OR=0.78; 95% CI, 0.64-0.97). In combined analysis, a borderline significance was also observed in subjects carrying the rs4938723 CT/CC and TP53 GG genotypes (adjusted OR=0.66; 95% CI, 0.43-0.99). These findings indicate that the rs4938723 in the promoter region of pri-miR-34b/c was a protective factor for the development of CRC. As the significance is marginal, further replication studies are warranted to confirm these results.
基金：
the Program
for Changjiang Scholars and Innovative Research
Team in University (IRT 0935) and the National Natural
Science Foundation of China (No. 30901720).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类|3 区生物
小类|3 区生化与分子生物学
最新[2023]版：
大类|4 区生物学
小类|4 区生化与分子生物学
第一作者：
第一作者机构：[1]Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education , Chengdu, Sichuan 610041 , P.R. China[2]Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children ’ s Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041 , P.R. China
共同第一作者：
通讯作者：
通讯机构：[1]Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education , Chengdu, Sichuan 610041 , P.R. China[*1]Laboratory of Molecular and Translational Medicine , West China Institute of Women and Children ’ s Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041 , P.R. China
推荐引用方式(GB/T 7714)：
Lin-Bo Gao,Li-Juan Li,Xin-Min Pan,et al.A genetic variant in the promoter region of miR-34b/c is associated with a reduced risk of colorectal cancer.[J].Biological chemistry.2013,394(3):415-20.doi:10.1515/hsz-2012-0297.
APA：
Lin-Bo Gao,Li-Juan Li,Xin-Min Pan,Zhao-Hui Li,Wei-Bo Liang...&Lin Zhang.(2013).A genetic variant in the promoter region of miR-34b/c is associated with a reduced risk of colorectal cancer..Biological chemistry,394,(3)
MLA：
Lin-Bo Gao,et al."A genetic variant in the promoter region of miR-34b/c is associated with a reduced risk of colorectal cancer.".Biological chemistry 394..3(2013):415-20
