机构：[1]Department of Forensic Biology, West China School of Preclinical and Forensic Medicine[2]Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children’s Health, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan[3]Central Laboratory, Yunnan University of Chinese Traditional Medicine, Kunming, Yunnan[4]Department of Thyroid and Breast Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, P.R. China四川大学华西医院
Tumor suppressor p53 directly regulated the abundance of the miR-34b/c. The interaction might contribute to certain cancer. We hypothesized that rs4938723 in the promoter region of pri-miR-34b/c and TP-53 Arg72Pro may be related to the risk of papillary thyroid carcinoma (PTC). A total of 784 patients with PTC and 1006 healthy controls were recruited to participate in this study. The variants were discriminated using a polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). Additionally, the relative expression levels of miR-34b/c and TP-53 in 44 paired samples were revealed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). A significantly increased risk of PTC was observed in the miR-34b/c rs4938723 CT, CC, and CT/CC genotypes compared with the TT genotype (CT vs TT: adjusted odds ratio [OR] = 1.51, 95%confidence interval [CI] = 1.23-1.85; CC vs TT: adjusted OR = 1.89, 95%CI = 1.39-2.63; CT/CC vs TT: adjusted OR = 1.59, 95%CI = 1.30-1.92, respectively). Significantly increased PTC susceptibility was also associated with the TP-53 Arg72Pro CC and CG/CC genotypes compared with the GG genotype (CC vs GG: adjusted OR = 2.04, 95%CI = 1.54-2.70; CG/CC vs GG: adjusted OR = 1.35, 95%CI = 1.11-1.67, respectively). Stratification analysis revealed that patients carrying the TP-53 Arg72Pro C allele and CC genotype had a significantly increased risk for developing N1 (C vs. G: OR = 1.27, 95%CI = 1.03-1.56; CC vs. GG: OR = 1.62, 95%CI = 1.07-2.46, respectively). Combined analysis showed that the genotypes of rs4938723 CT/CC + TP-53CG/CC increased the risk of PTC compared with rs4938723TT + TP-53GG (OR = 2.25, 95%CI = 1.67-3.03). Additionally, level of miR-34b was significantly upregulated in PTC patients.These findings indicate that the miR-34b/c rs4938723 and TP-53 Arg72Pro polymorphisms may contribute to the susceptibility of PTC.
基金：
the Special Research Foundation of
Doctoral Priority to the Development of Field Project (No.
20110181130013), National Natural Science Foundation of China
(No. 81302149, 81202387), the Science & Technology Pillar Program
of Sichuan Province (2014SZ0001, 2013JY0013), Distinguished Young
Scientist of Sichuan University (No. 2013SCU04A38), and the Ph.D.
Programs Foundation of Ministry of Education of China (No.
20130181120011).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|2 区医学
小类|2 区医学：内科
最新[2023]版：
大类|4 区医学
小类|4 区医学：内科
第一作者：
第一作者机构：[1]Department of Forensic Biology, West China School of Preclinical and Forensic Medicine[2]Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children’s Health, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan
共同第一作者：
通讯作者：
通讯机构：[1]Department of Forensic Biology, West China School of Preclinical and Forensic Medicine[2]Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children’s Health, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan[*1]Sichuan University Chengdu, Sichuan 610041, China
推荐引用方式(GB/T 7714)：
Chen Peng,Sun Ruifen,Pu Yan,et al.Pri-Mir-34b/C and Tp-53 Polymorphisms are Associated With The Susceptibility of Papillary Thyroid Carcinoma: A Case-Control Study.[J].Medicine.2015,94(38):e1536.doi:10.1097/MD.0000000000001536.
APA：
Chen Peng,Sun Ruifen,Pu Yan,Bai Peng,Yuan Fang...&Gao Linbo.(2015).Pri-Mir-34b/C and Tp-53 Polymorphisms are Associated With The Susceptibility of Papillary Thyroid Carcinoma: A Case-Control Study..Medicine,94,(38)
MLA：
Chen Peng,et al."Pri-Mir-34b/C and Tp-53 Polymorphisms are Associated With The Susceptibility of Papillary Thyroid Carcinoma: A Case-Control Study.".Medicine 94..38(2015):e1536
