机构：[1]Department of Hepatology, Xinqiao Hospital[2]Department of Anesthesiology, Southwest Hospital[3]State Key Laboratory of Trauma and Burns, Surgery Research Institute, and Department of Traumatic Surgery, Daping Hospital, Third Military Medical University, Chongqing[4]Department of Nephrology, People’s Liberation Army Chengdu Military Area Command General Hospital, Chengdu, Sichuan, China[5]Anaesthetics, PainMedicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, London, United Kingdom.
Blast limb injury was reported to result in distant organ injury including the lungs, which can be attenuated with transient regional hypothermia (RH) to the injured limb. We aimed to further study hepatic and renal injuries following blast limb trauma and also to evaluate the protective effects of regional traumatic limb hypothermia on such injuries in rats.
Blast limb trauma (BLT) was created using chartaceous electricity detonators in anesthetized male Sprague-Dawley rats. The BLT rats were randomly allocated to undergo regional traumatic limb hypothermic treatment (RH) for 30 minutes, 60 minutes, or 6 hours immediately after the onset of blast or without RH (n = 8 per group). The severity of hepatic and renal injury was assessed through histologic examination and water content (wet/dry weight) in all animals 6 hours later. The level of plasma tumor necrosis factor α (TNF-α), interleukin 6, hydrogen sulfide (H2S), and myeloperoxidase (MPO) together with hepatic and renal MPO, malondialdehyde (MDA), superoxide dismutase, and total antioxidant capacity were measured 6 hours after the blast injury.
Following BLT, hepatic injury was evidenced by histopathologic changes, increased water content, as well as plasma alanine aminotransferase and aspartate aminotransferase. Renal histopathologic but not functional changes were also found. RH treatment for all durations attenuated this distant renal injury, but only RH treatment for 60 minutes and 6 hours attenuated distant hepatic injury following BLT. RH treatment for all durations decreased plasma TNF-α and interleukin 6, reduced liver and kidney MPO activity and kidney MDA, and elevated superoxide dismutase and total antioxidant capacity in both liver and kidneys. RH treatment for 60 minutes is the most effective duration to reduce hepatic MPO activity, plasma TNF-α, and kidney MDA.
This study indicates that BLT-induced distant renal and hepatic injury could be attenuated by RH treatment through reduction of cytokine release and inhibition of neutrophil accumulation and oxidative stress.
基金：
This study was supported by China and National Natural Science
Foundation of China (Grant No. 81100055)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|3 区医学
小类|3 区外科4 区危重病医学
最新[2023]版：
大类|2 区医学
小类|2 区外科3 区危重病医学
第一作者：
第一作者机构：[1]Department of Hepatology, Xinqiao Hospital
共同第一作者：
通讯作者：
通讯机构：[3]State Key Laboratory of Trauma and Burns, Surgery Research Institute, and Department of Traumatic Surgery, Daping Hospital, Third Military Medical University, Chongqing[4]Department of Nephrology, People’s Liberation Army Chengdu Military Area Command General Hospital, Chengdu, Sichuan, China[*1]Department of Nephrology, People’s Liberation Army Chengdu Military Area, Command General Hospital, Chengdu, Sichuan,610083, China[*2]State Key Laboratory of Trauma and Burns, Surgery Research Institute, Department of Traumatic Surgery, Daping Hospital, Third Military Medical University., 10 Changjiang Rd, Chongqing, China
推荐引用方式(GB/T 7714)：
Zhao Hongzhi,Ning Jiaolin,Duan Jiaxiang,et al.Regional traumatic limb hypothermia attenuates distant hepatic and renal injury following blast limb trauma in rats.[J].The journal of trauma and acute care surgery.2014,77(3):464-70.doi:10.1097/TA.0000000000000396.
APA：
Zhao Hongzhi,Ning Jiaolin,Duan Jiaxiang,Gu Jianteng,Yi Bin...&Ma Daqing.(2014).Regional traumatic limb hypothermia attenuates distant hepatic and renal injury following blast limb trauma in rats..The journal of trauma and acute care surgery,77,(3)
MLA：
Zhao Hongzhi,et al."Regional traumatic limb hypothermia attenuates distant hepatic and renal injury following blast limb trauma in rats.".The journal of trauma and acute care surgery 77..3(2014):464-70
