Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro.
机构：[1]Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of China[2]State Key Laboratory of Oncogenes and R elated Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People’s Republic of China[3]Department of Ultrasound, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People’s Republic of China
The gene knockdown activity of small interfering RNA (siRNA) has led to their use as potential therapeutics for a variety of diseases. However, successful gene therapy requires safe and efficient delivery systems. In this study, we choose mPEG-PLGA-PLL nanoparticles (PEAL NPs) with ultrasound targeted microbubble destruction (UTMD) to efficiently deliver siRNA into cells. An emulsification-solvent evaporation method was used to prepare siRNA-loaded PEAL NPs. The NPs possessed an average size of 132.6±10.3 nm (n=5), with a uniform spherical shape, and had an encapsulation efficiency (EE) of more than 98%. As demonstrated by MTT assay, neither PEAL NPs nor siRNA-loaded PEAL NPs showed cytotoxicity even at high concentrations. The results of cellular uptake showed, with the assistance of UTMD, the siRNA-loaded PEAL NPs can be effectively internalized and can subsequently release siRNA in cells. Taken together, PEAL NPs with UTMD may be highly promising for siRNA delivery, making it possible to fully exploit the potential of siRNA-based therapeutics.
基金：
This research was supported by grants from the National
Natural Science Foundation of China (numbers 81201097,
81272568, 81472841, and 81301973) and the Nature Science
Foundation of Shanghai (number 12ZR1424800).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|2 区工程技术
小类|2 区药学3 区纳米科技
最新[2023]版：
大类|2 区医学
小类|2 区药学3 区纳米科技
第一作者：
第一作者机构：[1]Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of China[2]State Key Laboratory of Oncogenes and R elated Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of China[3]Department of Ultrasound, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People’s Republic of China[*1]Department of Ultrasound, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiaotong University, 2200/25 Xietu Road, Shanghai 200080, People’s Republic of China[*2]Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, No 17, the 3rd Section of People’s South Road, Chengdu 610041, Sichuan, People’s Republic of China
推荐引用方式(GB/T 7714)：
Yanwei Teng,Min Bai,Ying Sun,et al.Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro.[J].International journal of nanomedicine.2015,10:5447-57.doi:10.2147/IJN.S81172.
APA：
Yanwei Teng,Min Bai,Ying Sun,Qi Wang,Fan Li...&Yourong Duan.(2015).Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro..International journal of nanomedicine,10,
MLA：
Yanwei Teng,et al."Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro.".International journal of nanomedicine 10.(2015):5447-57
医学