机构：[1]Department of Stomatology, People’s Hospital of Mianzhu, Mianzhu, Sichuan, China[2]New Era Stroke Care and Research Institute, The PLA Second Artillery General Hospital, Beijing, China[3]Department of Oncology, Third People’s Hospital of Chengdu, Chengdu, Sichuan, China[4]Department of Centre for Disease Prevention and Control, Chengdu Military Region, Chengdu, Sichuan, China
Oral squamous cell carcinoma (OSCC) is the sixth most common human malignancy worldwide. To develop new therapeutics requires elucidation of the underlying mechanism of OSCC pathogenesis. The role of miR-429 in OSCC remains unknown.
The level of miR-429 and ZEB1 in OSCC tissues and cell lines was measured by qRT-PCR. MiR-429 was down-regulated by miRNAs antisense oligonucleotides (ASO) transfection and up-regulated by miRNAs mimics. Cell proliferation was analyzed by MTT assay. Cell apoptosis was revealed by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay.
MiR-429 was down-regulated in OSCC tissues, and miR-429 overexpression inhibited OSCC cell lines growth and vice versa. Further, we found that miR-429 could inhibit zinc finger E-boxbinding homeobox 1 (ZEB1) expression, and that miR-429 and ZEB1 expression in OSCC tissues were negatively correlated.
Our data demonstrate the tumor suppressor role of miR-429 in OSCC, and may provide a potential therapeutic target that warrants further investigation.
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外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区医学：研究与实验
最新[2023]版：
大类|4 区医学
小类|4 区医学：研究与实验
第一作者：
第一作者机构：[1]Department of Stomatology, People’s Hospital of Mianzhu, Mianzhu, Sichuan, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Lei Wanke,Liu Yun-e,Zheng Yuzhu,et al.MiR-429 inhibits oral squamous cell carcinoma growth by targeting ZEB1.[J].Medical science monitor : international medical journal of experimental and clinical research.2015,21:383-9.doi:10.12659/MSM.893412.
APA：
Lei Wanke,Liu Yun-e,Zheng Yuzhu&Qu Lin.(2015).MiR-429 inhibits oral squamous cell carcinoma growth by targeting ZEB1..Medical science monitor : international medical journal of experimental and clinical research,21,
MLA：
Lei Wanke,et al."MiR-429 inhibits oral squamous cell carcinoma growth by targeting ZEB1.".Medical science monitor : international medical journal of experimental and clinical research 21.(2015):383-9
