机构：[1]Sichuan Medical University, Luzhou, Sichuan, China[2]Department of Respiratory Medicine, Jiangjin District Institute of Respiratory Diseases, Jiangjin Central Hospital of Chongqing, Chongqing, China
The aim of this study was to investigate the correlation between the expression of matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) and clinicopathological features of lung adenocarcinoma. The expression of MMP-9 and VEGF was evaluated by immunohistochemistry of 30 samples from lung adenocarcinoma patients and 12 paratumoral (normal) tissue samples. In addition, the change in VEGF or MMP-9 expression after MMP-9 or VEGF blockade, respectively, was measured using western blot in lung adenocarcinoma A549 cells. High expression of MMP-9 was found in 63.3% of adenocarcinoma tissues versus 16.7% in normal tissues (P < 0.05). High expression of VEGF was found in 70% of adenocarcinoma tissues versus 25% in normal tissues (P < 0.05). A positive correlation was identified between MMP-9 and VEGF expression (correlation coefficient = 0.7094, P < 0.001), and their mutual overexpression was associated with clinical staging and lymph node status (P < 0.05). In addition, an decrease in VEGF protein expression was observed after MMP-9 blockade by an MMP-9-specific monoclonal antibody. Similarly, a decrease in MMP-9 protein expression was found after VEGF blockade by a VEGF-specific monoclonal antibody. In conclusion, VEGF and MMP-9 are overexpressed in lung adenocarcinoma tissues, and they have a synergistic effect on the invasion and metastasis of adenocarcinoma.
基金：
Research supported by the General Program of Natural Science Foundation of Chongqing
(#CSTC2011jjA0961).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区生物
小类|4 区生化与分子生物学4 区遗传学
最新[2023]版：
无
第一作者：
第一作者机构：[1]Sichuan Medical University, Luzhou, Sichuan, China
通讯作者：
通讯机构：[1]Sichuan Medical University, Luzhou, Sichuan, China[2]Department of Respiratory Medicine, Jiangjin District Institute of Respiratory Diseases, Jiangjin Central Hospital of Chongqing, Chongqing, China
推荐引用方式(GB/T 7714)：
Y.L. Wen,L. Li.Correlation between matrix metalloproteinase-9 and vascular endothelial growth factor expression in lung adenocarcinoma.[J].Genetics and molecular research : GMR.2015,14(4):19342-8.doi:10.4238/2015.December.29.44.
APA：
Y.L. Wen&L. Li.(2015).Correlation between matrix metalloproteinase-9 and vascular endothelial growth factor expression in lung adenocarcinoma..Genetics and molecular research : GMR,14,(4)
MLA：
Y.L. Wen,et al."Correlation between matrix metalloproteinase-9 and vascular endothelial growth factor expression in lung adenocarcinoma.".Genetics and molecular research : GMR 14..4(2015):19342-8
