The aim of the present study was to verify whether overexpression of CXC receptor 4 (CXCR4) promotes the invasion and migration of non-small cell lung cancer (NSCLC) via epidermal growth factor receptor (EGFR) and matrix metallopeptidase-9 (MMP-9), and to detect the association between CXCR4, EGFR and MMP-9. The effects of overexpression of CXCR4 on lung cancer cell functions were investigated by migration and invasion assays. Western blotting and zymograph assays were used to analyze the protein expression levels of EGFR and the production of MMP-9, respectively. Immunohistochemistry was applied to analyze the expression of EGFR, CXCR4 and MMP-9 in NSCLC. Statistical analyses were used to detect the associations among EGFR, CXCR4 and MMP-9 in NSCLC. Finally, survival analyses were performed. CXCR4 overexpression enhanced cell motility and invasion. CXCR4 also promoted expression of EGFR and elevated MMP-9 production. CXCR4, EGFR and MMP-9 were highly expressed in NSCLC, and were not identified as associated with age and sex (P>0.05). However, they were associated with tumor differentiation and lymph node metastasis (P<0.05). CXCR4, EGFR and CXCR4 expression were positively associated with one another in NSCLC (P<0.05). In addition, patients with positive expression of CXCR4, EGFR or MMP-9 in tumors exhibited significantly shorter overall survival compared with those with negative expression (P<0.05). In conclusion, CXCR4 overexpression enhanced cell motility and invasion via EGFR and MMP-9. CXCR4, EGFR and MMP-9 were identified as highly expressed in NSCLC, and there was positive correlation among them.
基金：
National Nature Science Foundation of ChinaNational Natural Science Foundation of China [81272960]; Key Research Program from Science and Technology Department of Hunan Province, China [2013WK2010]; Key Research Program from Ministry of Human Resources and Social Security of the People's Republic of China [2016176]; Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology [2016TP1015]; Construction Program of the Key Discipline in Hunan Province, China (Basic Medicine Sciences in University of South China) [201176]; General Research Program from Science and Technology Department of Hunan Province, China [2013FJ3145]; Funds for the Development of Chemical Industry of Forest Products in Hunan Province Key Laboratory [JDLC201203]; Wu Jieping Foundation Research Fund Project [320.6750.12245]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2023]版：
大类|4 区医学
小类|4 区肿瘤学
第一作者：
第一作者机构：[1]Univ South China, Sch Med, Canc Res Inst, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang, Hunan, Peoples R China
通讯作者：
通讯机构：[2]Univ South China, Affiliated Hosp 1, Dept Radiotherapy, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China[3]Wuhan Univ, Zhongnan Hosp, Dept Resp, Wuhan 430071, Hubei, Peoples R China[4]Peoples Hosp Zhangjiajie City, Dept Resp & Crit Care Med, 192 Guyong Rd, Zhangjiajie 427000, Hunan, Peoples R China
推荐引用方式(GB/T 7714)：
Zuo Jianhong,Wen Meiling,Li Sai,et al.Overexpression of CXCR4 promotes invasion and migration of non-small cell lung cancer via EGFR and MMP-9[J].ONCOLOGY LETTERS.2017,14(6):7513-7521.doi:10.3892/ol.2017.7168.
APA：
Zuo, Jianhong,Wen, Meiling,Li, Sai,Lv, Xiu,Wang, Lei...&Lei, Mingsheng.(2017).Overexpression of CXCR4 promotes invasion and migration of non-small cell lung cancer via EGFR and MMP-9.ONCOLOGY LETTERS,14,(6)
MLA：
Zuo, Jianhong,et al."Overexpression of CXCR4 promotes invasion and migration of non-small cell lung cancer via EGFR and MMP-9".ONCOLOGY LETTERS 14..6(2017):7513-7521
