机构:[1]The Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.重庆医科大学附属第一医院[2]The Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.[3]Institute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.[4]The Department of Pathology, North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.[5]The Department of Medical Microbiology and Parasitology, North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.
MicroRNAs have recently emerged as regulators of many biological processes including cell proliferation, development and differentiation. This study identified that miR-22 was statistically decreased in colorectal cancer clinical specimens and highly metastatic cell lines. Moreover, low miR-22 expression was associated with tumor metastasis, advanced clinical stage and relapse. Consistent with clinical observations, miR-22 significantly suppressed the ability of colorectal cancer cells to growth and metastasize in vitro and in vivo. Sp1 was validated as a target of miR-22, and ectopic expression of Sp1 compromised the inhibitory effects of miR-22. In addition, Sp1 repressed miR-22 transcription by binding to the miR-22 promoter, hence forming a negative feedback loop. Further study has shown that miR-22 suppresses the activity of PTEN/AKT pathway by Sp1. Our present results implicate the newly indentified miR-22/Sp1/PTEN/AKT axis might represent a potential therapeutic target for colorectal cancer.
基金:
This study was supported by Scientific Research Project of Sichuan Medical Association (S15024), Sichuan Youth Science and Technology Foundation (2017JQ0039). Scientific Research Project of Nanchong Municipal Science and Technology Bureau (16YFZJ0128), Scientific Research Project of Sichuan Provincial Health and Family Planning Commission (16ZD037), and Scientific Research Fund of Sichuan Provincial Education Department of China (14ZA0184, 13ZA0230).
第一作者机构:[1]The Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
共同第一作者:
通讯作者:
通讯机构:[2]The Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.[3]Institute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.
推荐引用方式(GB/T 7714):
Xia Shu-Sen,Zhang Guang-Jun,Liu Zuo-Liang,et al.MicroRNA-22 suppresses the growth, migration and invasion of colorectal cancer cells through a Sp1 negative feedback loop.[J].ONCOTARGET.2017,8(22):36266-36278.doi:10.18632/oncotarget.16742.
APA:
Xia Shu-Sen,Zhang Guang-Jun,Liu Zuo-Liang,Tian Hong-Peng,He Yi...&Zhou Tong.(2017).MicroRNA-22 suppresses the growth, migration and invasion of colorectal cancer cells through a Sp1 negative feedback loop..ONCOTARGET,8,(22)
MLA:
Xia Shu-Sen,et al."MicroRNA-22 suppresses the growth, migration and invasion of colorectal cancer cells through a Sp1 negative feedback loop.".ONCOTARGET 8..22(2017):36266-36278