机构：[1]Department of Thoracic Oncology, State Key Laboratory of Biotherapy and Cancer Centre, West China Hospital, Sichuan University, Chengdu, China.四川大学华西医院[2]Institute of Clinical Pharmacology, GCP Center, West China Hospital, Sichuan University, Chengdu, China.四川大学华西医院[3]Department of Oncology (Section Three), Affiliated Hospital of Zunyi Medical University, Zunyi, China.[4]Department of Oncology (Section One), Affiliated Hospital of Zunyi Medical University, Zunyi, China.
It has been observed that lncRNAs have been taking part in many cancer progressions, including non-small cell lung cancer and gastric cancer. Meanwhile, lncRNA small nucleolar RNA host gene 22 (SNHG22) has been studied, taking part in the progression of ovarian epithelial carcinoma. However, we know little about the function of SNHG22 in esophageal squamous cell carcinoma (ESCC).In this study, we will explore the inner mechanism of SNHG22 in ESCC. Quantitative real-time PCR (qRT-PCR) assay was implemented in ESCC cells for detecting the expression of lncRNA, SNHG22, and miR-429. Also, functional experiments, including CCK8 and colony formation assay, were implemented to assess the growth of ESCC cells. Meanwhile, flow cytometry analysis was conducted to test the apoptosis of ESCC cells. The immunofluorescence (IF) assay and western blot were conducted to verify the autophagy of ESCC cells.
Inhibition of SNHG22 was found that can inhibit the progression and promotes autophagy and apoptosis of ESCC cells. Meanwhile, as subcellular fraction assay and FISH assay found that SNHG22 mainly in the cytoplasm, miR-429 was found can bind to SNHG22 and SESN3 by RIP assay and luciferase reporter assay. SESN3 was found it can play the oncogene in ESCC cells.
SNHG22 promotes the progression of ESCC by the miR-429/SESN3 axis.
2020 Annals of Translational Medicine. All rights reserved.
基金：
This work was supported by National Natural Science Foundation of China Grants (no. 81860441) and Guizhou Province Science and Technology Cooperation Grants {no. LH[2015]7486}.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区医学
小类|3 区肿瘤学3 区医学：研究与实验
最新[2023]版：
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第一作者：
第一作者机构：[1]Department of Thoracic Oncology, State Key Laboratory of Biotherapy and Cancer Centre, West China Hospital, Sichuan University, Chengdu, China.[2]Institute of Clinical Pharmacology, GCP Center, West China Hospital, Sichuan University, Chengdu, China.[3]Department of Oncology (Section Three), Affiliated Hospital of Zunyi Medical University, Zunyi, China.
通讯作者：
通讯机构：[1]Department of Thoracic Oncology, State Key Laboratory of Biotherapy and Cancer Centre, West China Hospital, Sichuan University, Chengdu, China.[2]Institute of Clinical Pharmacology, GCP Center, West China Hospital, Sichuan University, Chengdu, China.[*1]Department of Thoracic Oncology, State Key Laboratory of Biotherapy and Cancer Centre, West China Hospital, Chengdu, China[*2]University and Collaborative Innovation Centre of Biotherapy, Chengdu, China
推荐引用方式(GB/T 7714)：
Li Zhong-Wen,Zhang Ting-You,Yue Guo-Jun,et al.Small nucleolar RNA host gene 22 (SNHG22) promotes the progression of esophageal squamous cell carcinoma by miR-429/SESN3 axis.[J].Annals of translational medicine.2020,8(16):1007.doi:10.21037/atm-20-5332.
APA：
Li Zhong-Wen,Zhang Ting-You,Yue Guo-Jun,Tian Xin,Wu Jin-Zhi...&Wang Yong-Sheng.(2020).Small nucleolar RNA host gene 22 (SNHG22) promotes the progression of esophageal squamous cell carcinoma by miR-429/SESN3 axis..Annals of translational medicine,8,(16)
MLA：
Li Zhong-Wen,et al."Small nucleolar RNA host gene 22 (SNHG22) promotes the progression of esophageal squamous cell carcinoma by miR-429/SESN3 axis.".Annals of translational medicine 8..16(2020):1007
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