机构：[1]College of Chemistry, Sichuan University, Chengdu 610064, China[2]Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, China[3]Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China
Copper-64 is a useful radioisotope for positron emission tomography (PET). Due to the wide range of applications, the demand of 64Cu with low metallic impurities is increasing. Here we report a simple method for the efficient production of high specific activity 64Cu using a cyclotron for biomedical application. We designed new equipment based on the plating of enriched 64Ni as the target, and used automated ion exchange chromatography to purify copper-64 efficiently after irradiation and dissolution of the target in good radiochemical and chemical yield and purity. The 64Cu radionuclide produced using 99.32% enriched 64Ni with a density of 61.4 ± 5.0 mg/cm², reaching a total radioactivity greater than 200 mCi, with specific activity up to 5.6 GBq/μmoL. It was further incorporated into modified monoclonal antibody DOTA-rituximab to synthesize 64Cu-DOTA-rituximab, which was used successfully for micro-PET imaging.
基金：
This work was supported, in part, by the National Natural Science Foundation of China
(No. 81371592, No. 81401467, No. 81501519, No. 81571705 and No. 81671733), Beijing Natural Science Foundation
(No. 7162041 and No. 7171002), Beijing Municipal Commission of Health and Family Planning (215 backbone
program), and 2017 Beijing Science & Technology Nova Star Program (xx2017016).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区化学
小类|3 区有机化学
最新[2023]版：
大类|2 区化学
小类|3 区生化与分子生物学3 区化学：综合
第一作者：
第一作者机构：[1]College of Chemistry, Sichuan University, Chengdu 610064, China[2]Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Qinghua Xie,Hua Zhu,Feng Wang,et al.Establishing Reliable Cu-64 Production Process: From Target Plating to Molecular Specific Tumor Micro-PET Imaging.[J].Molecules (Basel, Switzerland).2017,22(4):doi:10.3390/molecules22040641.
APA：
Qinghua Xie,Hua Zhu,Feng Wang,Xiangxi Meng,Qiushi Ren...&Zhi Yang.(2017).Establishing Reliable Cu-64 Production Process: From Target Plating to Molecular Specific Tumor Micro-PET Imaging..Molecules (Basel, Switzerland),22,(4)
MLA：
Qinghua Xie,et al."Establishing Reliable Cu-64 Production Process: From Target Plating to Molecular Specific Tumor Micro-PET Imaging.".Molecules (Basel, Switzerland) 22..4(2017)
