机构：[1]The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou 515041, Guangdong, People’s Republic of China[2]Department of Biochemistry and Molecular Biology, Shantou University Medical College, 22 Xinling Road, Shantou 515041, Guangdong, People’s Republic of China[3]Institute of Oncologic Pathology, Shantou University Medical College, Shantou 515041, Guangdong, People’s Republic of China[4]Medical Bioinformatics Center, Shantou University Medical College, Shantou 515041, Guangdong, People’s Republic of China[5]Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, People’s Republic of China四川大学华西医院
Lysyl oxidase-like 4 (LOXL4), a member of the LOX family proteins, catalyzes oxidative deamination of lysine residues in collagen and elastin, which are responsible for maintaining extracellular matrix homeostasis. In this study, the mRNA expression of LOXL4 in seven esophageal squamous cell carcinoma (ESCC) cell lines and 15 ESCC pairs of clinical samples were examined. Furthermore, LOXL4 protein levels in the ESCC cell lines were determined using western blotting. With the use of immunofluorescence, LOXL4 was observed to be localized primarily in the cytoplasm, but was also present in the nucleus. In addition, the results indicated that the upregulated expression of LOXL4 was associated with poor survival in patients with ESCC even following curative resection (P = 0.010). Similar Kaplan-Meier estimator curves for proteins that interact with LOXL4, SUV39H1 (P = 0.014) and COL2A1 (P = 0.011), were plotted. The analyses based on the protein-protein interaction network depicted the expression of LOXL4 and its associated proteins as well as their functions, suggesting that LOXL4 and its associated proteins may serve a significant role in the development and progression of ESCC. In conclusion, the results of the present study suggest that LOXL4 is a potential biomarker for patients with ESCC, as well as SUV39H1 and COL2A1, and high expression levels of these genes are associated with poor prognosis in patients with ESCC.
基金：
National Natural
Science Foundation of China (Nos. 81472613 and 81360331), the
Natural Science Foundation of China-GuangDong Joint Fund (Nos.
U1301227 and U1601229), the Science & Technology Planning Project
of GuangDong Province (No. 2014A020212286), the Natural Science
Foundation of Guangdong Province (No. 2018A030313664).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|3 区生物学
小类|3 区生化与分子生物学
最新[2023]版：
大类|3 区生物学
小类|3 区生化与分子生物学
第一作者：
第一作者机构：[1]The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou 515041, Guangdong, People’s Republic of China[2]Department of Biochemistry and Molecular Biology, Shantou University Medical College, 22 Xinling Road, Shantou 515041, Guangdong, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou 515041, Guangdong, People’s Republic of China[2]Department of Biochemistry and Molecular Biology, Shantou University Medical College, 22 Xinling Road, Shantou 515041, Guangdong, People’s Republic of China
推荐引用方式(GB/T 7714)：
Xie Weijie,Huang Peiqi,Wu Bingli,et al.Clinical significance of LOXL4 expression and features of LOXL4-associated protein-protein interaction network in esophageal squamous cell carcinoma.[J].Amino acids.2019,51(5):813-828.doi:10.1007/s00726-019-02723-4.
APA：
Xie Weijie,Huang Peiqi,Wu Bingli,Chen Sijie,Huang Zijian...&Zou Haiying.(2019).Clinical significance of LOXL4 expression and features of LOXL4-associated protein-protein interaction network in esophageal squamous cell carcinoma..Amino acids,51,(5)
MLA：
Xie Weijie,et al."Clinical significance of LOXL4 expression and features of LOXL4-associated protein-protein interaction network in esophageal squamous cell carcinoma.".Amino acids 51..5(2019):813-828
