机构：[1]Department of Gynecology and Obstetrics, Center of Reproductive Medicine, The West China Second University Hospital, Sichuan University, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, China[2]Department of Gynecology and Obstetrics, The West China Second University Hospital, Sichuan University, Chengdu, China[3]Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children’s Health, The West China Second University Hospital, Sichuan University, Chengdu, China[4]National Office for Maternal and Child Health Surveillance of China, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), The West China Second University Hospital, Sichuan University, Ministry of Education, Chengdu, China[5]Department of Pathology, The West China Second University Hospital, Sichuan University, Chengdu, China
To compare long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression levels in endometrium between patients with repeated implantation failure (RIF) following in vitro fertilization (IVF)-embryo transfer and control women.
RNA sequencing (RNA-seq) and alignments were performed to identify lncRNAs and mRNAs using endometrial samples collected from 3 patients and 3 control women. A subset of 10 differentially expressed lncRNAs and 6 mRNAs were validated in all participants using quantitative reverse transcription polymerase chain reaction. The potential biological roles of identified lncRNAs were predicted via coexpressed mRNA annotations. Twenty patients with RIF and 30 control women were recruited for validation.
We identified 1202 differentially expressed genes, including 742 lncRNAs and 460 mRNAs, in mid-secretory phase endometrial tissue from patients with RIF following IVF compared to control women. We analyzed the target genes of the lncRNAs and uncovered 148 lncRNAs corresponding to 147 cis-regulated target genes. The cis-regulated target genes of these significantly differentially expressed lncRNAs were clustered into several pathways, such as the tumor necrosis factor signaling pathway, the Toll-like receptor signaling pathway, and the NF-kappa B (NF-κB) signaling pathway.
Our study constitutes the first report on the investigation of the regulatory mechanisms of lncRNAs in endometrial receptivity in women experiencing RIF using RNA-seq. Our results provide a valuable candidate reservoir for future functional studies of lncRNAs.
基金：
the National Natural Science Foundation
of China (no 81671422).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|3 区医学
小类|3 区妇产科学4 区生殖生物学
最新[2023]版：
大类|3 区医学
小类|4 区妇产科学4 区生殖生物学
第一作者：
第一作者机构：[1]Department of Gynecology and Obstetrics, Center of Reproductive Medicine, The West China Second University Hospital, Sichuan University, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, China
通讯作者：
通讯机构：[1]Department of Gynecology and Obstetrics, Center of Reproductive Medicine, The West China Second University Hospital, Sichuan University, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, China[*1]Department of Gynecology and Obstetrics, Center of Reproductive Medicine, The West China Second University Hospital, Sichuan University, Ministry of Education, Chengdu, China
推荐引用方式(GB/T 7714)：
Chen Min-Yan,Liao Guang-Dong,Zhou Bin,et al.Genome-Wide Profiling of Long Noncoding RNA Expression Patterns in Women With Repeated Implantation Failure by RNA Sequencing.[J].Reproductive sciences (Thousand Oaks, Calif.).2019,26(1):18-25.doi:10.1177/1933719118756752.
APA：
Chen Min-Yan,Liao Guang-Dong,Zhou Bin,Kang Le-Ni,He Yan-Mei&Li Shang-Wei.(2019).Genome-Wide Profiling of Long Noncoding RNA Expression Patterns in Women With Repeated Implantation Failure by RNA Sequencing..Reproductive sciences (Thousand Oaks, Calif.),26,(1)
MLA：
Chen Min-Yan,et al."Genome-Wide Profiling of Long Noncoding RNA Expression Patterns in Women With Repeated Implantation Failure by RNA Sequencing.".Reproductive sciences (Thousand Oaks, Calif.) 26..1(2019):18-25
