机构：[1]State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, 14#, 3rd Section of Renmin South Road, Chengdu 610041, China[2]Department of Intensive Care Unit, Sichuan Cancer Hospital & Research Institute, Chengdu, China四川省肿瘤医院
As a gamma-secretase inhibitor, DAPT has been widely used to evaluate the biological behaviors and Notch signaling pathway in various cells This study was aimed to examine the effects of DAPT on the growth and vitamin D-3 induced osteogenesis in adipose derived stem cells (ASCs). The cells were treated with or without DART and induced to osteoblastic lineage in the presence of vitamin D-3 Alizarin red staining and real-time PCR results indicated that the addition of DAPT to vitamin D-3 treatments enhanced osteogenesis in ASCs. According to the fold increase and colony-forming unit assay results, the cells cultured in DAPT exhibited lower proliferation rate than those cultured in control medium Hey1, expressed in the nucleus of ASCs to act as a transcriptional repressor, was downregulated when Notch signaling was inhibited by DAPT. Whereas the expression of Runx2 increased in the nucleus of osteogenic induced ASCs after DART treatment This study demonstrated that DAPT reduced the proliferation and enhanced the osteogenesis in ASCs via regulation of Notch and Runx2 expression (C) 2010 Elsevier Inc All rights reserved
基金：
State Key Laboratory of Oral Diseases Funds [GZZXJF-KOU-2-ZZXM-3, SKLODPI006]; National Basic Research Program of China [2006CB708505, 2010CB944800]; NSFC Funding of China [30901688, 30801304]
第一作者机构：[1]State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, 14#, 3rd Section of Renmin South Road, Chengdu 610041, China
通讯作者：
推荐引用方式(GB/T 7714)：
Jing Wei,Xiong Zhonghua,Cai Xiaoxiao,et al.Effects of gamma-secretase inhibition on the proliferation and vitamin D-3 induced osteogenesis in adipose derived stem cells[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2010,392(3):442-447.doi:10.1016/j.bbrc.2010.01.043.
APA：
Jing, Wei,Xiong, Zhonghua,Cai, Xiaoxiao,Huang, Yuanding,Li, Xiaoyu...&Tian, Weidong.(2010).Effects of gamma-secretase inhibition on the proliferation and vitamin D-3 induced osteogenesis in adipose derived stem cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,392,(3)
MLA：
Jing, Wei,et al."Effects of gamma-secretase inhibition on the proliferation and vitamin D-3 induced osteogenesis in adipose derived stem cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 392..3(2010):442-447
