Under normal conditions, circadian rhythms of rodents are derived from the suprachiasmatic nucleus (SCN) and primarily entrained by light. But food-related signals, such as restricted feeding (RF), can also affect circadian rhythms and result in food-entrained locomotor activities in mice, suggesting that an additional oscillating circadian pacemaker besides the SCN is responsible for this regulation. However, little is known about its detailed molecular mechanisms. Our study found that RF during subjective day under continuous darkness augmented mice wheel-running locomotors during the RF period. Additionally, the orexin-A (OXA) neuron activity was increased obviously, and the mRNA and protein levels of RACK1 were significantly elevated. The activation of OXA neurons was prior to the initiation of RF and the elevation of RACK1. These results suggest that OXA and RACK1 may be involved in wheel-running locomotor activities entrained by RF during subjective day in mice.