机构：[a]Department of Pediatric Respiratory, West China Second University Hospital, Sichuan University, Chengdu 610041, China[b]Department of Respiratory, Chengdu Women & Children's Hospital, Chengdu 610091, China[c]The Vascular Remodeling and Developmental Defects Research Unit, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu 610041, China[d]Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China[e]Department of Basic Research, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China四川省人民医院四川省肿瘤医院[f]State Key Laboratory of Molecular Oncology, Department of Aetiology and Carcinogenesis, Cancer Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China[g]Laboratory of Lung Development and Diseases, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu 610041, China
Background: Leukotrienes play critical roles in many inflammatory lung diseases and several antagonists of their receptors have been used in the clinical settings. However, the physiological functions of leukotrienes in lung development are still unclear. Method: The expression levels of 34 genes involved in leukotriene synthesis and function pathway in the lungs of Rhesus monkey during different developmental time points were determined on a MiSeq platform and analyzed by the reads per kilobase of transcript per million mapped reads (RPKM) method. Results: The results showed that the expression levels of PLA2G1B, PLA2G10, PLA2G2D, ALOX5, and ALOX5AP increased dramatically in the lung of Rhesus monkey, reflecting the changes in the pulmonary environment after delivery. Additionally, the different expression patterns between molecules related to LTB4 and LTC4 synthesis suggested distinct roles of LTB4 and LTC4 in lung development. Finally, the constant expression of CysLT1 during the development process provided new information to the pharmaceutical basis of the use of leukotriene receptor antagonists in the clinical setting. Conclusion The expression levels of several key genes involved in leukotriene synthesis changed dramatically during lung development in Rhesus monkeys, suggesting the potential roles of leukotrienes in lung development in this animal model.
基金：
This study was supported by the Support Program of
Science & Technology Department of Sichuan Province (No.
2013SZ0040) and Research Fund for the Doctoral Program of Higher
Education of China, Ministry of Education of China (No.
20130181110056).
第一作者机构：[a]Department of Pediatric Respiratory, West China Second University Hospital, Sichuan University, Chengdu 610041, China[b]Department of Respiratory, Chengdu Women & Children's Hospital, Chengdu 610091, China
共同第一作者：
通讯作者：
通讯机构：[a]Department of Pediatric Respiratory, West China Second University Hospital, Sichuan University, Chengdu 610041, China[c]The Vascular Remodeling and Developmental Defects Research Unit, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu 610041, China[d]Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China[g]Laboratory of Lung Development and Diseases, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu 610041, China[*1]Laboratory of Lung Development and Diseases, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu 610041, China.[*2]Department of Pediatric Respiratory, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
推荐引用方式(GB/T 7714)：
Wanmin Xia,Liang Xie,Bangrong Cao,et al.Genes involved in leukotriene synthesis pathway are dynamically regulated during lung development in Rhesus monkeys[J].PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS.2017,122:1-6.doi:10.1016/j.plefa.2017.06.008.
APA：
Wanmin Xia,Liang Xie,Bangrong Cao,Shujun Cheng,Huajing Wan&Hanmin Liu.(2017).Genes involved in leukotriene synthesis pathway are dynamically regulated during lung development in Rhesus monkeys.PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS,122,
MLA：
Wanmin Xia,et al."Genes involved in leukotriene synthesis pathway are dynamically regulated during lung development in Rhesus monkeys".PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS 122.(2017):1-6
