This study tried to quantify spinal cord perfusion by using contrast-enhanced ultrasound (CEUS) in rhesus monkey models with acute spinal cord injury. Acute spinal cord perfusion after injury was detected by CEUS, coupling with conventional ultrasound (US) and Color Doppler US (CDFI). Time-intensity curves and perfusion parameters were obtained by autotracking contrast quantification (ACQ) software in the epicenter and adjacent regions of injury, respectively. Neurological and histological examinations were performed to confirm the severity of injury. US revealed spinal cords were hypoechoic and homogeneous, whereas dura maters, pia maters, and cerebral aqueducts were hyperechoic. After spinal cord contusion, the injured spinal cord was hyperechoic on US, and intramedullary vessels of adjacent region of injury were increased and dilated on CDFI. On CEUS hypoperfusion were found in the epicenter of injury, while hyperperfusion in its adjacent region. Quantitative analysis showed that peak intensity (PI) decreased in epicenters of injury but significantly increased in adjacent regions at all time points (p < 0.05). Functional evaluation demonstrated significant deterioration compared to pre-contusion (p < 0.05). Quantitative analysis with CEUS is a promising method for monitoring perfusion changes of spinal cord injury in overall views and real-time.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30901506]; Provincial Natural Science Foundation of Guangdong [2010B060500021, 10151008901000155, 8151008901000094]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|2 区医学
小类|2 区肿瘤学3 区细胞生物学
最新[2025]版：
无
第一作者：
第一作者机构：[1]Sun Yat Sen Univ, Mem Hosp, Dept Orthoped, Inst Spinal Cord Injury, Guangzhou 510120, Guangdong, Peoples R China;
通讯作者：
通讯机构：[1]Sun Yat Sen Univ, Mem Hosp, Dept Orthoped, Inst Spinal Cord Injury, Guangzhou 510120, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Dept Ultrasound, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Guangdong, Peoples R China
推荐引用方式(GB/T 7714)：
Huang Lin,Chen Keng,Chen Fu-Chao,et al.Intraoperative contrast-enhanced ultrasonography for microcirculatory evaluation in rhesus monkey with spinal cord injury[J].ONCOTARGET.2017,8(25):40756-40764.doi:10.18632/oncotarget.17252.
APA：
Huang, Lin,Chen, Keng,Chen, Fu-Chao,Shen, Hui-Yong,Ye, Ji-Chao...&Lin, Xi.(2017).Intraoperative contrast-enhanced ultrasonography for microcirculatory evaluation in rhesus monkey with spinal cord injury.ONCOTARGET,8,(25)
MLA：
Huang, Lin,et al."Intraoperative contrast-enhanced ultrasonography for microcirculatory evaluation in rhesus monkey with spinal cord injury".ONCOTARGET 8..25(2017):40756-40764
