机构：[a]Department of Dermatology, West China Hospital, Sichuan University, Chengdu, Sichuan, China四川大学华西医院[b]Cancer Center, The Second Clinical Medical College of North Sichuan Medical College, Nanchong Central Hospital, Nanchong, Sichuan, China[c]Department of Breast Surgery, Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu, Sichuan, China四川省人民医院[d]Department of Chemotherapy, Sichuan Cancer Hospital, Chengdu, Sichuan, China四川省肿瘤医院[e]Department of Pathology, Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu, Sichuan, China四川省人民医院[f]Cancer Center, Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu, Sichuan, China四川省人民医院
Background and Aims. Jagged1/Notch3 signaling pathway plays a key role in angiogenesis of breast cancer, but little is known in TNBC. This study was designed to investigate the expression of Jagged1/Notch3 mRNA and protein in TNBC, analyze their correlations with clinicopathological characteristics and prognosis. Moreover, the interrelationship among Jagged1/Notch3 and VEGF was initially evaluated. Methods. Jagged1/Notch3 mRNA and protein expression levels were determined by QRT-PCR and Western blotting. Additionally, Immunohistochemistry for Jagged1/Notch3 was detected by Ventana platform, VEGF and CD34 was performed using the EnVision/HRP technique. Results. mRNA transcriptionof Jagged1/Notch3 was in accord with protein expression. TNBC patients with positive Jagged1 expression had poorer DFS (p = 0.008) and OS (p = 0.004). Jagged1 expression was independent predictors of OS (p = 0.038). The expression of VEGF was positively correlative to MVD (p = 0.018), MVD was significantly associated with Jagged1 (p <0.0001) and Notch3 (p <0.0001). The expression of Jagged1/Notch3 has no correlation with VEGF, only in positive VEGF expression of TNBC patients Jagged1/Notch3 had influence on DFS and OS (p <0.05). Conclusion. Jagged1/Notch3 was -expressed at both the mRNA and protein levels, Jagged1 served as an independent predictor of poor prognosis. We speculate that there is a cross -talk between Jagged1/Notch3 and VEGF in TNBC angiogenesis. Jagged1/Notch3 is expected to be an important signaling pathway for TNBC progression and a potential target for TNBC neovascularization therapy. (C) 2017 IMSS. Published by Elsevier Inc.
基金：
This work was supported by the National Natural Science Foundation
of China (81502747) and Youth Fund of Sichuan provincial people’s hospital (30305030605).
第一作者机构：[a]Department of Dermatology, West China Hospital, Sichuan University, Chengdu, Sichuan, China
共同第一作者：
通讯作者：
通讯机构：[f]Cancer Center, Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu, Sichuan, China[*1]Cancer Center, Academy of Medical Sciences and Sichuan Provincial People’s Hospital, No. 32, West Second Section First Ring Road, Chengdu 610072, Sichuan, China
推荐引用方式(GB/T 7714)：
Siliang Xue,Lang He,Xiao Zhang,et al.Expression of Jagged1/Notch3 Signaling Pathway and their Relationship with the Tumor Angiogenesis in TNBC[J].ARCHIVES OF MEDICAL RESEARCH.2017,48(2):169-179.doi:10.1016/j.arcmed.2017.03.014.
APA：
Siliang Xue,Lang He,Xiao Zhang,Jin Zhou,Fanghua Li&Xiaoshan Wang.(2017).Expression of Jagged1/Notch3 Signaling Pathway and their Relationship with the Tumor Angiogenesis in TNBC.ARCHIVES OF MEDICAL RESEARCH,48,(2)
MLA：
Siliang Xue,et al."Expression of Jagged1/Notch3 Signaling Pathway and their Relationship with the Tumor Angiogenesis in TNBC".ARCHIVES OF MEDICAL RESEARCH 48..2(2017):169-179
