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SP1-induced upregulation of long non-coding RNA HCP5 promotes the development of osteosarcoma

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收录情况： ◇ SCIE

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机构： [1]Food Nutrition Center, West China Hospital, Sichuan University, Chengdu, 610041, No. 37, Guoxue Xiang, Wuhou District, Sichuan, China [2]Department of Lymphoma, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chendu, 610041, No. 55 Section 4 South Renmin Road, Sichuan, China

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关键词： SP1 LncRNA HCP5 Invasion EMT Osteosarcoma

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Long non-coding RNAs (LncRNAs) are acknowledged as crucial regulators in tumorigenesis and tumor progression. In this study, we explored the mechanism and function of lncRNA HCP5 in osteosarcoma (OS). At first, five lncRNAs were chosen from GeneCard and subjected to qRT-PCR examination. The results indicated that HCP5 was significantly overexpressed in four OS cell lines. Northern blot assay further proved the higher expression of HCP5 in OS cell lines. To identify the biological role of HCP5 in OS, we silenced the expression of HCP5 in U2OS and MG-63 cells which possessed the highest level of HCP5. CCK-8 and colony formation assay revealed the inhibitory effect of HCP5 knockdown on cell proliferation. Cell apoptosis was found to be increased in cells transfected with sh-HCP5#1. Moreover, cell invasion and epithelial-mesenchymal transition (EMT) were reversed by the silencing of HCP5. The results of functional assays showed that HCP5 acted as an oncogene in osteosarcoma. Mechanically, HCP5 was found to be activated by the transcription factor SP1. Finally, rescue assays were conducted to demonstrate the function of SP1/HCP5 axis in osteosarcoma. In conclusion, we confirmed that SP1-induced upregulation of long non-coding RNA HCP5 promotes the development of osteosarcoma.

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出版当年[2019]版：

大类 | 4 区医学

小类 | 4 区病理学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区病理学

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出版当年[2019]版：

Q3 PATHOLOGY

最新[2023]版：

Q2 PATHOLOGY

影响因子： 2.9 最新[2023版] 2.6 最新五年平均 2.05 出版当年[2019版] 1.801 出版当年五年平均 1.794 出版前一年[2018版] 3.25 出版后一年[2020版]

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第一作者机构： [1]Food Nutrition Center, West China Hospital, Sichuan University, Chengdu, 610041, No. 37, Guoxue Xiang, Wuhou District, Sichuan, China

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