Simultaneous multielement analysis by ICP-MS with simple whole blood sample dilution and its application to uremic patients undergoing long-term hemodialysis
机构：[1]Department of Clinical Laboratory, Mianyang Central Hospital, Mianyang, Sichuan, China[2]Department of Pharmacy, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China临床药学部临床药学部四川省人民医院四川省肿瘤医院[3]Department of Pharmacy, Evidence-Based Pharmacy Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China
Few studies were reported on trace elements' alterations in uremic patients undergoing long-term (>24 months) hemodialysis (HD), especially by using the whole blood as the biological fluid for the measuring purpose. Our objective was to develop an improved micro-sampling inductively coupled plasma-mass spectrometry (ICP-MS) method to determine the levels of Ca, Mg, Cu, Zn, Fe, Mn, Se and Pb in uremic patients receiving long-term HD. A ICP-MS method with a modified whole blood sample preparation procedure with small volumes was established and applied for the simultaneous quantification of the various elements in uremic patients undergoing long-term HD. 124 eligible uremic patients receiving long-term HD (75 males and 49 females) and 77 healthy subjects (54 males and 23 females) were recruited and Ca, Mg, Cu, Zn, Fe, Mn, Se and Pb levels were further determined. Our results revealed that uremic patients with HD had significantly higher blood levels of Ca, Mg, Zn and Pb and lower Cu, Fe, Se and Mn concentrations than healthy controls. In conclusion, a reproducible and reliable ICP-MS method using minimal whole blood sample volume (50 mu L) with a simple dilution-based preparation procedure was successfully improved, validated and applied. Uremic patients undergoing long-term HD might be at increased risk of some essential trace elements deficiency (especially for Cu, Fe and Se) or toxic trace element excess (Pb) in respect to healthy subjects. Monitoring of blood levels and supplementation of some trace elements may be indicated in uremic patients undergoing long-term HD.
基金：
Science and technology sup-port project of Sichuan Province [2015SZ0117]; Cadre Health Care Research Project of Sichuan Province [2019-801]; Zambon Pharmaceutical Scientific Research Foundation of Chengdu Pharmaceutical Association [201905]
第一作者机构：[1]Department of Clinical Laboratory, Mianyang Central Hospital, Mianyang, Sichuan, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, Mianyang Central Hospital, Mianyang, Sichuan, China[*1]Department of Clinical Laboratory, Mianyang Central Hospital, No. 12 Changjiaxiang, Jingzhong Street, Mianyang, Sichuan, 621000, China
推荐引用方式(GB/T 7714)：
Xu Bei,Zhang Yamei,Chen Yan,et al.Simultaneous multielement analysis by ICP-MS with simple whole blood sample dilution and its application to uremic patients undergoing long-term hemodialysis[J].SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION.2020,80(3):247-255.doi:10.1080/00365513.2020.1729401.
APA：
Xu, Bei,Zhang, Yamei,Chen, Yan,Zeng, Min,Feng, Jiafu...&Yu, Lin.(2020).Simultaneous multielement analysis by ICP-MS with simple whole blood sample dilution and its application to uremic patients undergoing long-term hemodialysis.SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION,80,(3)
MLA：
Xu, Bei,et al."Simultaneous multielement analysis by ICP-MS with simple whole blood sample dilution and its application to uremic patients undergoing long-term hemodialysis".SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION 80..3(2020):247-255
