详情页 - 四川省肿瘤医院知识库

当前位置：首页 > 详情页

Downregulation of long non-coding RNA MEG3 in nasopharyngeal carcinoma

文献详情

资源类型：

作者：

机构： [a]State Key Laboratory of Oncology in South China, Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China [b]Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, HKSAR, China [c]Li Ka Shing Faculty of Medicine, School of Biomedical Science and Center for Cancer Research, The University of Hong Kong, HKSAR, China [d]Partner State Key Laboratory of Digestive Disease, Institute of Digestive Disease, Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, Hong Kong

出处：

DOI：

ISSN：

关键词： lncRNA MEG3 NPC promoter hypermethylation tumor suppression

摘要：

In our previous whole-transcriptome sequencing analysis, downregulation of a long non-coding RNA, maternally expressed gene 3 (MEG3), was identified in NPC samples. This finding suggests the possible role of MEG3 as a tumor suppressor in this distinctive disease. In the present study, two MEG3 variants, AF119863 (MEG3-AF) and BX247998 (MEG3-BX), were found abundantly expressed in a normal nasopharyngeal epithelial cell line, NP69. Significant downregulation of MEG3-AF was further verified in a panel of NPC samples including xenografts and primary biopsies. MEG3 is an imprinted gene located within chromosome 14q32, a common deleted region in NPC. Both DNA copy number loss and aberrant promoter methylation contributed to MEG3 inactivation. Interestingly, MEG3 expression could successfully be rescued by the treatment of a demethylation agent. Besides, ectopic expression of MEG3 in NPC cell lines resulted in considerable repression of in vitro anchorage-independent growth and in vivo tumorigenicity, in addition to significant inhibition in cell proliferation, colony formation, and induction of cell cycle arrest. Finally, we revealed the association between MEG3 activity and the p53 signaling cascade. Our findings characterize MEG3 as a tumor suppressive long non-coding RNA in NPC and encourage the development of precise long non-coding RNA-targeted epigenetic therapy against this malignancy. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

语种：

中科院(CAS)分区：

出版当年[2017]版：

大类 | 2 区医学

小类 | 2 区生化与分子生物学 2 区肿瘤学

最新[2023]版：

大类 | 2 区医学

小类 | 2 区生化与分子生物学 3 区肿瘤学

第一作者：

第一作者机构： [a]State Key Laboratory of Oncology in South China, Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis. [2]lncRNA MEG3 inhibits the growth of hepatocellular carcinoma cells by sponging miR-9-5p to upregulate SOX11. [3]Critical Role of Myeloid-Derived Suppressor Cells in Tumor-Induced Liver Immune Suppression through Inhibition of NKT Cell Function. [4]Metallic Nanoparticle-Doped Oxide Semiconductor Film for Bone Tumor Suppression and Bone Regeneration [5]CXCL8 in Tumor Biology and Its Implications for Clinical Translation. [6]Tumor-derived microparticles promote the progression of triple-negative breast cancer via PD-L1-associated immune suppression. [7]Receptor-type tyrosine-protein phosphatase κ directly targets STAT3 activation for tumor suppression in nasal NK/T-cell lymphoma. [8]Study of Cetuximab in Nasopharyngeal Carcinoma (NPC) With Chemoradiotherapy [9]LncRNA KCNQ1OT1在非小细胞肺癌组织中的表达及其临床意义 [10]Individualized Elective Neck Irradiation in NPC Patients