By the method of 1H NMR prescreening and tracing the diagnostic signals, 6-methylpyridinone derivatives, (8R,9S)-dihydroisoflavipucine (1), (8S,9S)-dihydroisoflavipucine (2), 3- (1-hydr oxy-4-methy1-2- oxopentylidene)-6-methylpyridine-2, 4(1 H,3H)-di one (3), 4-hydr oxy-3(2-hydr oxy-4-methylpentanoy1)-6-methylpyridin-2(1 H)- one (4), 4-hydroxy-3-[(4-hydr oxy-6-methyl2- oxo-2H-pyran-3-yemethy1]-6-methylpyridin-2(1H)- one (5) and a known 6-methylpyranone derivative 3,3'-methylenebis(4-hydroxy-6-methyl-2H-pyran-2-one) (6) were isolated from the marine fungus Leptosphaerulina sp., which was collected from the starfish Acanthaster planci from the South China Sea. Compounds 2 and 3 are new compounds. Their structures were elucidated on the basis of MS, 1D and 2D NMR, and X-ray single crystal diffraction data. The absolute configurations of compounds 1 and 2 were determined by analysis on the experimental circular-dichroism spectra. (C) 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.