机构：[1]West China Hospital/West China School of Nursing, Sichuan University, Chengdu 610041, China.四川大学华西医院[2]Department of Gastroenterology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.[3]Institute for Disaster Management and Reconstruction, Sichuan University, Chengdu 610207, China.[4]College of Biomass Science and Engineering, Key Laboratory of Biomass Fibers for Medical Care in Textile Industry, Sichuan University, Chengdu 610065, China.[5]Research Institute for Intelligent Wearable Systems and Research Centre of Textiles for Future Fashion, School of Fashion and Textiles, The Hong Kong Polytechnic University, Kowloon, Hong Kong 999077, China.[6]Department of Clinical Nutrition, West China Second Hospital, Sichuan University, Chengdu 610041, China.[7]Department of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.[8]Sate Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
In response to increasing antibiotic resistance and the pressing demand for safer infected wound care, probiotics have emerged as promising bioactive agents. To address the challenges associated with the safe and efficient application of probiotics, this study successfully loaded metabolites from Lacticaseibacillus rhamnosus GG (LGG) into a gelatin cross-linked macromolecular network by an in situ blending and photopolymerization method. The obtained LM-GelMA possesses injectability and autonomous healing capabilities. Importantly, the incorporation of LGG metabolites endows LM-GelMA with excellent antibacterial properties against Staphylococcus aureus and Escherichia coli, while maintaining good biocompatibility. In vivo assessments revealed that LM-GelMA can accelerate wound healing by mitigating infections induced by pathogenic bacteria. This is accompanied by a reduction in the expression of key proinflammatory cytokines such as TNF-α, IL-6, VEGFR2, and TGF-β, leading to increased re-epithelialization and collagen formation. Moreover, microbiological analysis confirmed that LM-GelMA can modulate the abundance of beneficial wound microbiota at family and genus levels. This study provides a facile strategy and insights into the functional design of hydrogels from the perspective of wound microenvironment regulation.
基金：
This work was financially sponsored by the National Natural
Science Foundation of China (U22A20334), Sichuan Science
and Technology Programs (2022JDRC0087), the Sichuan
University Innovative Research Project (2023SCUH0032), the
West China Nursing Discipline Development Special Fund
Project (HXHL21025, HXHL21027), and the Sichuan
University Postdoctoral Interdisciplinary Innovation Fund
(10822041A2087, JCXK2231).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|2 区化学
小类|1 区有机化学1 区高分子科学2 区生化与分子生物学
最新[2023]版：
大类|2 区化学
小类|1 区有机化学1 区高分子科学2 区生化与分子生物学
第一作者：
第一作者机构：[1]West China Hospital/West China School of Nursing, Sichuan University, Chengdu 610041, China.
共同第一作者：
通讯作者：
通讯机构：[4]College of Biomass Science and Engineering, Key Laboratory of Biomass Fibers for Medical Care in Textile Industry, Sichuan University, Chengdu 610065, China.[8]Sate Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
推荐引用方式(GB/T 7714)：
Han Yanting,Yin Zhe,Wang Yilin,et al.Photopolymerizable and Antibacterial Hydrogels Loaded with Metabolites from Lacticaseibacillus rhamnosus GG for Infected Wound Healing[J].Biomacromolecules.2024,doi:10.1021/acs.biomac.4c00124.
APA：
Han Yanting,Yin Zhe,Wang Yilin,Jiang Yuanzhang,Chen Jianming...&Li Ka.(2024).Photopolymerizable and Antibacterial Hydrogels Loaded with Metabolites from Lacticaseibacillus rhamnosus GG for Infected Wound Healing.Biomacromolecules,,
MLA：
Han Yanting,et al."Photopolymerizable and Antibacterial Hydrogels Loaded with Metabolites from Lacticaseibacillus rhamnosus GG for Infected Wound Healing".Biomacromolecules .(2024)
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