机构:[1]Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Guangdong, Peoples R China;其他部门华南肿瘤学国家重点实验室中山大学肿瘤防治中心[2]Karolinska Inst, Dept Med Epidemiol & Biostat, Stockholm, Sweden;[3]Harvard Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA;[4]Stanford Univ, Sch Med, Dept Hlth Res & Policy, Div Epidemiol, Stanford, CA USA;[5]Sihui Canc Inst, Sihui, Guangdong, Peoples R China
Genetic susceptibility and Epstein-Barr virus (EBV) infection are important etiological factors in nasopharyngeal carcinoma (NPC). In this study, in southern China, where NPC is endemic, a single nucleotide polymorphism (SNP) in the EBV-encoded RPMS1 gene (locus 155391: G > A [G155391A]) and seven host SNPs (rs1412829, rs28421666, rs2860580, rs2894207, rs31489, rs6774494, and rs9510787) were confirmed to be significantly associated with NPC risk in 50 NPC cases versus 54 hospital-based controls with throat washing specimens and 1925 NPC cases versus 1947 hospital-based controls with buffy coat samples, respectively. We established a strategy to detect the NPC-associated EBV and host SNPs using saliva samples in a single test that is convenient, noninvasive, and cost-effective and displays good compliance. The potential utility of this strategy was tested by applying a risk prediction model integrating these EBV and host genetic variants to a population-based case-control study comprising 1026 incident NPC cases and 1148 controls. Receiver operating characteristic (ROC) curve analysis revealed an area under the curve of the NPC risk prediction model of 0.74 (95% CI: 0.71-0.76). Net reclassification improvement (NRI) analysis showed that inclusion of the EBV SNP significantly improved the discrimination ability of the model (NRI = 0.30, P < 0.001), suggesting the promising value of EBV characteristics for identifying high-risk NPC individuals in endemic areas. Taken together, we developed a promising NPC risk prediction model via noninvasive saliva sampling. This approach might serve as a convenient and effective method for screening the population with high-risk of NPC.
基金:
National Basic Research Program of ChinaNational Basic Research Program of China [2011CB504302]; National High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2012AA02A501]; US National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01CA115873-01]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81222035, 81430059]
语种:
外文
被引次数:
WOS:
PubmedID:
中科院(CAS)分区:
出版当年[2017]版:
大类|2 区医学
小类|2 区肿瘤学3 区细胞生物学
最新[2023]版:
无
第一作者:
第一作者机构:[1]Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Guangdong, Peoples R China;
通讯作者:
通讯机构:[1]Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Guangdong, Peoples R China;[2]Karolinska Inst, Dept Med Epidemiol & Biostat, Stockholm, Sweden;[3]Harvard Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA;
推荐引用方式(GB/T 7714):
Cui Qian,Feng Fu-Tuo,Xu Miao,et al.Nasopharyngeal carcinoma risk prediction via salivary detection of host and Epstein-Barr virus genetic variants[J].ONCOTARGET.2017,8(56):95066-95074.doi:10.18632/oncotarget.11144.
APA:
Cui, Qian,Feng, Fu-Tuo,Xu, Miao,Liu, Wen-Sheng,Yao, You-Yuan...&Zeng, Yi-Xin.(2017).Nasopharyngeal carcinoma risk prediction via salivary detection of host and Epstein-Barr virus genetic variants.ONCOTARGET,8,(56)
MLA:
Cui, Qian,et al."Nasopharyngeal carcinoma risk prediction via salivary detection of host and Epstein-Barr virus genetic variants".ONCOTARGET 8..56(2017):95066-95074