机构：[1]The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China.[2]The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.[3]Department of Pathology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China.[4]Department of Breast Oncology, Sun Yat-sen University Cancer Center,State Key Laboratory of Oncology in South China,Collaborative Innovation Center of Cancer Medicine, Guangzhou, China.临床科室其他部门乳腺科华南肿瘤学国家重点实验室中山大学肿瘤防治中心[5]Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
Backgroud: Accumulating evidences indicate that circular RNAs (circRNAs), a class of non-coding RNAs, play important roles in tumorigenesis. However, the function of circRNAs in triple negative breast cancer (TNBC) is largely unknown. Methods: We performed circRNA microarrays to identify circRNAs that are aberrantly expressed in TNBC cell lines. Expression levels of a significantly upregulated circRNA, circGFRA1, was detected by quantitative real-time PCR (qRTPCR) in TNBC cell lines and tissues. Kaplan-Meier survival analysis was used to explore the significance of circGFRA1 in clinical prognosis. Then, we examined the functions of circGFRA1 in TNBC by cell proliferation, apoptosis and mouse xenograft assay. In addition, luciferase assay was used to explore the miRNA sponge function of circGFRA1 in TNBC. Results: Microarray analysis and qRT-PCR verified a circRNA termed circGFRA1 that was upregulated in TNBC. KaplanMeier survival analysis showed that upregulated circGFRA1 was correlated with poorer survival. Knockdown of circGFRA1 inhibited proliferation and promoted apoptosis in TNBC. Via luciferase reporter assays, circGFRA1 and GFRA1 was observed to directly bind to miR-34a. Subsequent experiments showed that circGFRA1 and GFRA1 regulated the expression of each other by sponging miR-34a. Conclusions: Taken together, we conclude that circGFRA1 may function as a competing endogenous RNA (ceRNA) to regulate GFRA1 expression through sponging miR-34a to exert regulatory functions in TNBC. circGFRA1 may be a diagnostic biomarker and potential target for TNBC therapy.
基金：
National Natural Science Foundation of China [81,672,683, 81,772,961]; Science and Technology Planning Projects of Guangdong [2016A020214009]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|2 区医学
小类|2 区肿瘤学
最新[2023]版：
大类|1 区医学
小类|2 区肿瘤学
第一作者：
第一作者机构：[1]The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China.[2]The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.[3]Department of Pathology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China.
通讯作者：
通讯机构：[1]The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China.[2]The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.[4]Department of Breast Oncology, Sun Yat-sen University Cancer Center,State Key Laboratory of Oncology in South China,Collaborative Innovation Center of Cancer Medicine, Guangzhou, China.
推荐引用方式(GB/T 7714)：
He Rongfang,Liu Peng,Xie Xiaoming,et al.circGFRA1 and GFRA1 act as ceRNAs in triple negative breast cancer by regulating miR-34a[J].JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH.2017,36(1):-.doi:10.1186/s13046-017-0614-1.
APA：
He, Rongfang,Liu, Peng,Xie, Xiaoming,Zhou, Yujuan,Liao, Qianjin...&Tang, Hailin.(2017).circGFRA1 and GFRA1 act as ceRNAs in triple negative breast cancer by regulating miR-34a.JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,36,(1)
MLA：
He, Rongfang,et al."circGFRA1 and GFRA1 act as ceRNAs in triple negative breast cancer by regulating miR-34a".JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 36..1(2017):-
医学