机构：[1]Department of Infectious Disease, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China[2]Department of Tuberculosis, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China[3]Infection and Immune Laboratory, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China[4]MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou, Guangdong 510632, China[5]MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University First Affiliated Hospital, Guangzhou 510632, China[6]Antiviral Therapy Center, the First People’s Hospital of Yuexi County, Liangshan 616650, China
Objective This study aimed to investigate the molecular transmission network and drug resistance in treatment-naïve HIV-1 infected patients in the Liangshan District, China. Methods The research subjects for this study were HIV-1 infected patients who did not receive any anti-retroviral therapy (ART) in the Liangshan District between January 2022 and July 2023. Peripheral venous whole blood samples were collected from the research subjects. 2 mL blood was used for CD4+ T lymphocyte counting detection. 10 mL blood was centrifuged to separate the plasma and blood cells for quantitative detection of HIV-1 RNA and DNA and drug resistance testing of HIV-1. Results A total of 156 participants were included in this study (88 males and 68 females). The median age of the participants was 37 years old. The findings revealed a positive correlation between the HIV-1 DNA and the HIV-1 RNA levels (r=0.478, P<0.001). However, a negative correlation was observed between HIV-1 DNA levels and CD4+ T lymphocyte counts (r=-0.186, P=0.020). Of the 156 participants, 148 were successfully tested for drug resistance of HIV-1 RNA and HIV-1 DNA simultaneously. Four cases failed the HIV-1 RNA drug resistance testing, and another four failed the HIV-1 DNA drug resistance testing. The most common HIV-1 subtype was the CRF07_BC recombinant. In this study, the overall incidence of transmitted drug resistance (TDR) was 8.33%. The resistance rates of non-nucleoside reverse transcriptase inhibitor (NNRTI) and protease inhibitor (PI) were 7.69% and 0.64%, respectively. Additionally, 32 participants were found to have drug-resistant mutations. The primary drug-resistant mutations were K103N, V179D, E157Q, and A128T, mainly against efavirenz (EFV) and nevirapine (NVP) resistance. Conclusion The drug resistance of HIV-1 infected ART-naïve patients in the Liangshan District cannot be ignored. HIV-1 drug resistance testing is recommended before initiating ART.
基金：
This study was supported by Chinese Association of STD & AIDS Prevention and Control·Maternal and Child Tigermed Care and Prevention of Maternal to Child Transmission Fund (PMTCT202207); Liangshan Science and Technology Program (23ZDYF0064) and Doctoral Research Initiation Fund of Affiliated Hospital of Southwest Medical University.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2024]版：
无
最新[2023]版：
大类|4 区医学
小类|4 区免疫学4 区传染病学4 区病毒学
第一作者：
第一作者机构：[1]Department of Infectious Disease, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China[2]Department of Tuberculosis, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China[3]Infection and Immune Laboratory, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Infectious Disease, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China[2]Department of Tuberculosis, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China[3]Infection and Immune Laboratory, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China[*1]Department of Tuberculosis, Infection and Immune Laboratory, the Affiliated Hospital of Southwest Medical University, No.25 Taiping Street, Luzhou, Sichuan, 646000, China.
推荐引用方式(GB/T 7714)：
Cao Bianchuan,Wu Caihong,Liu Mei,et al.Molecular transmission network and drug resistance in treatment-naïve HIV-1 infected patients in the Liangshan District, China[J].AIDS Research And Human Retroviruses.2024,doi:10.1089/AID.2024.0016.
APA：
Cao Bianchuan,Wu Caihong,Liu Mei,Song Shaofang,Wu Tao...&Zhong Li.(2024).Molecular transmission network and drug resistance in treatment-naïve HIV-1 infected patients in the Liangshan District, China.AIDS Research And Human Retroviruses,,
MLA：
Cao Bianchuan,et al."Molecular transmission network and drug resistance in treatment-naïve HIV-1 infected patients in the Liangshan District, China".AIDS Research And Human Retroviruses .(2024)
