机构：[1]Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education of China), School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, China[2]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China[3]Department of Blood Transfusion, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, China四川省肿瘤医院[4]State Key Laboratory for Mesoscopic Physics and Frontiers Science Centre for Nano-optoelectronics, School of Physics, Peking University, Beijing 100871, China[5]School of Electrical Engineering and Telecommunications, University of New South Wales, Sydney, NSW 2052, Australia[6]Department of Clinical Laboratory, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, China四川省肿瘤医院
Multiplexed biomarker detection is highly desirable for the early diagnosis and treatment of diseases. Optofluidic laser has shown extraordinary ability in single biomarker detection, but the simultaneous detection of multiple biomarkers remains unexplored. Here, we develop optofluidic laser immunosensors based on multidimensional division multiplexing technologies. Time division multiplexing (TDM) based optofluidic lasers are demonstrated by the temporal control of the immunoreaction and sequential generation of immunocomplexes. Spatial/ wavelength division multiplexing (SDM/WDM) based optofluidic lasers are achieved by integrating microfluidic channels and exploiting different emission bands. The feasibility of the multiplexed laser immunosensors is demonstrated by the detection of dual-biomarkers of acute myocardial infarction and lung cancer. The multiplexed laser immunosensor features the superimposed enhancement effects of laser amplification and latex particles, enabling a limit of detection down to the fM (10-15 molar) level. The assay time can be shortened to 15 min thanks to the utilization of homogeneous immunoreaction. Based on the multiplexed detection of biomarkers, our technology takes a significant step toward the early diagnosis of major diseases.
基金：
National Natural Science Foundation of China [62275043, 62205007, 61875034]; Fundamental Research Funds for the Central Universities [ZYG-X2021YGCX007]; The 111 Project [B14039]; Shenzhen Science and Technology Program [JCYJ20190808162603577]; Sichuan Science and Technology Program [2021YJ0101]; China Postdoctoral Science Foundation [2021M700208]
第一作者机构：[1]Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education of China), School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, China[2]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
通讯作者：
通讯机构：[1]Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education of China), School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, China[3]Department of Blood Transfusion, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, China[4]State Key Laboratory for Mesoscopic Physics and Frontiers Science Centre for Nano-optoelectronics, School of Physics, Peking University, Beijing 100871, China[*1]No. 55, Section 4, Renmin South Road, Chengdu, Sichuan 610041, China.[*2]No. 5, Yiheyuan Road, Haidian District, Beijing 100871, China.[*3]No. 2006, Xiyuan Ave., High-Tech Zone (West), Chengdu, Sichuan 611731, China
推荐引用方式(GB/T 7714)：
Liu Yiling,Zhang Yaxin,Wang Chenxiang,et al.Multiplexed optofluidic laser immunosensor for sensitive and rapid detection of biomarkers[J].SENSORS AND ACTUATORS B-CHEMICAL.2024,403:doi:10.1016/j.snb.2023.135198.
APA：
Liu, Yiling,Zhang, Yaxin,Wang, Chenxiang,Wang, Yanqiong,Zhang, Ke...&Gong, Yuan.(2024).Multiplexed optofluidic laser immunosensor for sensitive and rapid detection of biomarkers.SENSORS AND ACTUATORS B-CHEMICAL,403,
MLA：
Liu, Yiling,et al."Multiplexed optofluidic laser immunosensor for sensitive and rapid detection of biomarkers".SENSORS AND ACTUATORS B-CHEMICAL 403.(2024)
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