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Identification of polyunsaturated fatty acids as potential biomarkers of osteoarthritis after sodium hyaluronate and mesenchymal stem cell treatment through metabolomics

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机构: [1]School of Pharmacy, Macau University of Science and Technology, Taipa, Macao SAR, China. [2]State Key Laboratory for Quality Research of Chinese Medicine, Macau University of Science and Technology, Taipa, Macao SAR, China. [3]Liver Transplant Center and HBP Surgery, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
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关键词: osteoarthritis sodium hyaluronate mesenchymal stem cells metabolomics derivatization–liquid chromatography–mass spectrometry correlation analysis

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Introduction: Osteoarthritis (OA) is a prevalent joint disorder worldwide. Sodium hyaluronate (SH) and mesenchymal stem cells (MSCs) are promising therapeutic strategies for OA. Previous studies showed they could improve knee function and clinical symptoms of OA. However, the mechanism of the therapeutic effects on the improvement of OA has not been clearly explained. Methods: In our study, we used a technique called 5-(diisopropylamino)amylamine derivatization liquid chromatography coupled with mass spectrometry to find the metabolites in OA synovial fluid under different treatments. Results and Discussion: After looking into the metabolomics, we discovered that SH and MSC treatment led to the downregulation of ω-6 polyunsaturated fatty acids (PUFAs) and the upregulation of ω-3 PUFAs. Significantly, the contents of 5(S)-HETE, PGA2, PGB2, and PGJ2 were lower in the MSC group than in the SH group after quantification using 5-(diisopropylamino)amylamine derivatization-UHPLC-QQQ-MS. This is the first report on the relationship of 11(S)-HETE, PGA2, PGB2, PGF2β, 11β-PGF2α, and DK-PGE2 with OA. Moreover, the correlation analysis of metabolites and inflammation factors showed the positive association of ω-6 PUFAs with pro-inflammation cytokines, and of ω-3 PUFAs with anti-inflammation cytokines. Our results indicated the therapeutic effect of SH and MSCs in patients with OA. In addition, this reliable metabolic approach could uncover novel biomarkers to treat OA.Copyright © 2023 Yang, Zhao, Li, Wu, Huang, Zhang, Bian and Zhu.

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出版当年[2023]版:
大类 | 2 区 医学
小类 | 2 区 药学
最新[2023]版:
大类 | 2 区 医学
小类 | 2 区 药学
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出版当年[2023]版:
Q1 PHARMACOLOGY & PHARMACY
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Q1 PHARMACOLOGY & PHARMACY

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第一作者机构: [1]School of Pharmacy, Macau University of Science and Technology, Taipa, Macao SAR, China. [2]State Key Laboratory for Quality Research of Chinese Medicine, Macau University of Science and Technology, Taipa, Macao SAR, China. [3]Liver Transplant Center and HBP Surgery, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
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通讯机构: [1]School of Pharmacy, Macau University of Science and Technology, Taipa, Macao SAR, China. [2]State Key Laboratory for Quality Research of Chinese Medicine, Macau University of Science and Technology, Taipa, Macao SAR, China.
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