机构：[1]Department of Life Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire SL5 7PY, UK.[2]West China Biomedical Big Data Center, West China Hospital/West China School of Medicine, Sichuan University, Chengdu 610041, PR China.四川大学华西医院[3]Department of Genetics, University Medical Center Groningen, University of Groningen, Groningen 9713 AV, Netherlands.[4]Division of Molecular Pathology, The Institute of Cancer Research, London SM2 5NG, UK.[5]Changping Laboratory, Beijing 102206, PR China.[6]01Life Institute, Shenzhen 518000, PR China.[7]HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, PR China.[8]Department of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen 9713AV, Netherlands.[9]Department of Biology, University of Oxford, 11a Mansfield Rd, Oxford OX1 3SZ, UK.
Gut microbiomes in infancy have a profound impact on health in adulthood. CRISPRs play an essential role in the interaction between bacteria and phages. However, the dynamics of CRISPRs in gut microbiomes during early life are poorly understood. In this study, using shotgun metagenomic sequencing data from 82 Swedish infants' gut microbiomes, 1882 candidate CRISPRs were identified, and their dynamics were analysed. We found large-scale turnover of CRISPRs and their spacers during the first year of life. As well as changes in relative abundance of the bacteria containing CRISPR, acquisition, loss and mutation of spacers were observed within the same CRISPR array sampled over time. Accordingly, the inferred interaction network of bacteria and phage was distinct at different times. This research underpins CRISPR dynamics and their potential role in the interaction between bacteria and phage in early life.
基金：
This research was supported by CSC 202108510037, Dutch Heart Foundation IN-CONTROL
(CVON2018-27),
the ERC Consolidator grant (grant agreement
No. 101001678), NWO-VICI
grant (VI.C.202.022), and the Netherlands Organ-on-
Chip
Initiative, an NWO Gravitation project (024.003.001) funded
by the Ministry of Education, Culture and Science of the government of The Netherlands.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|2 区生物学
小类|2 区遗传学2 区微生物学
最新[2023]版：
大类|2 区生物学
小类|2 区遗传学2 区微生物学
第一作者：
第一作者机构：[1]Department of Life Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire SL5 7PY, UK.[2]West China Biomedical Big Data Center, West China Hospital/West China School of Medicine, Sichuan University, Chengdu 610041, PR China.[3]Department of Genetics, University Medical Center Groningen, University of Groningen, Groningen 9713 AV, Netherlands.
通讯作者：
通讯机构：[1]Department of Life Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire SL5 7PY, UK.[2]West China Biomedical Big Data Center, West China Hospital/West China School of Medicine, Sichuan University, Chengdu 610041, PR China.[3]Department of Genetics, University Medical Center Groningen, University of Groningen, Groningen 9713 AV, Netherlands.[9]Department of Biology, University of Oxford, 11a Mansfield Rd, Oxford OX1 3SZ, UK.
推荐引用方式(GB/T 7714)：
Wu Jiqiu,Zhang Hanyun,Gan Rui,et al.CRISPR dynamics during the interaction between bacteria and phage in the first year of life[J].Microbial genomics.2023,9(7):doi:10.1099/mgen.0.001053.
APA：
Wu Jiqiu,Zhang Hanyun,Gan Rui,Xia Yan,Zhang Fan...&Barraclough Timothy G.(2023).CRISPR dynamics during the interaction between bacteria and phage in the first year of life.Microbial genomics,9,(7)
MLA：
Wu Jiqiu,et al."CRISPR dynamics during the interaction between bacteria and phage in the first year of life".Microbial genomics 9..7(2023)
