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Phospholipase A2 induces acute kidney injury by complement mediated mitochondrial apoptosis via TNF-α/NF-κB signaling pathway

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机构: [1]Department of Nephrology and Kidney Research Institute, West China Hospital, Sichuan University, Chengdu, China
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关键词: Phospholipase A2 Wasp/bee stings Acute kidney injury Complement Apoptosis

摘要:
Acute kidney injury (AKI) is one of common complications of wasp/bee stings. Phospholipase A2 (PLA2) is a vital pathogenic composition of wasp/bee venom. We aimed to investigate the role of complement mediated mitochondrial apoptosis in PLA2 induced AKI.PLA2 induced AKI model was established by injecting PLA2 into via tail vein on mice. The pathological changes and the microstructural changes of kidney, complement activation, inflammation and apoptosis were detected in vitro and in vivo respectively.The results showed that PLA2 induced AKI models were successfully established in vivo and vitro. Compared with control, serum creatinine and urea nitrogen levels were elevated. Complement system activation and mitochondrial damage were observed. Expressions of IL-6, TNF-α, cleaved caspase-3 and cleaved caspase-9, and Bax/Bcl-2 increased in PLA2 induced AKI models. TNF-α/NF-κB signaling pathway activation in AKI models.In the present study, PLA2 induced AKI model was first successfully established to our knowledge. The role of complement mediated mitochondrial apoptosis pathway in renal tubular epithelial cells in PLA2 induced AKI were verified in vitro and vivo.Copyright © 2022. Published by Elsevier Ltd.

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出版当年[2023]版:
大类 | 3 区 医学
小类 | 3 区 食品科技 3 区 毒理学
最新[2023]版:
大类 | 3 区 医学
小类 | 3 区 食品科技 3 区 毒理学
第一作者:
第一作者机构: [1]Department of Nephrology and Kidney Research Institute, West China Hospital, Sichuan University, Chengdu, China
通讯作者:
通讯机构: [1]Department of Nephrology and Kidney Research Institute, West China Hospital, Sichuan University, Chengdu, China [*1]Department of Nephrology and Kidney Research Institute, West China Hospital, Sichuan University, No. 37 Guoxue lane, Wuhou district, Sichuan, Chengdu, 610041, China
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