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Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa

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资源类型：
Pubmed体系：
作者：
Hu Xueli[1] ; Wu Mingbo[1] ; Ma Teng[1] ; Zhang Yige[1] ; Zou Chaoyu[1] ; Wang Ruihuan[1] ; Zhang Yongxin[1] ; Ren Yuan[1,2] ; Li Qianqian[1] ; Liu Huan[1] ; Li Heyue[1] ; Wang Taolin[1] ; Sun Xiaolong[1] ; Yang Yang[1] ; Tang Miao[1] ; Li Xuefeng[3] ; Li Jing[2] ; Gao Xiang[4] ; Li Taiwen[2,*2] ; Zhou Xikun[1,*1] ;
机构： [1]State Key Laboratory of Biotherapy and Cancer Center West China Hospital Sichuan University and Collaborative Innovation Center for Biotherapy Chengdu China. [2]State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases Chinese Academy of Medical Sciences Research Unit of Oral Carcinogenesis and Management West China Hospital of Stomatology Sichuan University Chengdu China. [3]Department of Radiation Oncology The University of Texas MD Anderson Cancer Center Houston Texas USA. [4]Department of Neurosurgery and Institute of Neurosurgery State Key Laboratory of Biotherapy and Cancer Center West China Hospital West China Medical School Sichuan University and Collaborative Innovation Center for Biotherapy Chengdu China.
出处：
DOI： 10.1002/mco2.193

关键词： Pseudomonas aeruginosa pulmonary infection single-cell RNA sequencing

摘要：
Knowledge of the changes in the immune microenvironment during pulmonary bacterial acute and chronic infections is limited. The dissection of immune system may provide a basis for effective therapeutic strategies against bacterial infection. Here, we describe a single immune cell atlas of mouse lungs after acute and chronic Pseudomonas aeruginosa infection using single-cell transcriptomics, multiplex immunohistochemistry, and flow cytometry. Our single-cell transcriptomic analysis revealed large-scale comprehensive changes in immune cell composition and high variation in cell-cell interactions after acute and chronic P. aeruginosa infection. Bacterial infection reprograms the genetic architecture of immune cell populations. We identified specific immune cell types, including Cxcl2+ B cells and interstitial macrophages, in response to acute and chronic infection, such as their proportions, distribution, and functional status. Importantly, the patterns of immune cell response are drastically different between acute and chronic infection models. The distinct molecular signatures highlight the importance of the highly dynamic cell-cell interaction process in different pathological conditions, which has not been completely revealed previously. These findings provide a comprehensive and unbiased immune cellular landscape for respiratory pathogenesis in mice, enabling further understanding of immunologic mechanisms in infection and inflammatory diseases.© 2022 The Authors. MedComm published by Sichuan International Medical Exchange & Promotion Association (SCIMEA) and John Wiley & Sons Australia, Ltd.

基金：
National Natural Science Foundation of China, Grant/Award Numbers: 81922042, 82072999, 82172285; National Clinical Research Center for Geriatrics,West China Hospital, Sichuan University, Grant/Award Number: Z2018B02;Excellent Young Scientist Foundation of Sichuan University, Grant/Award Number: 2017SCU04A16; Innovative Spark Foundation of Sichuan University, Grant/Award Number: 2018SCUH0032; National Major Scientific and Technological Special Project for “Significant New Drugs Development”, Grant/Award Number: 2018ZX09201018-013; 1⋅3⋅5 project of excellent development of discipline of West China Hospital of Sichuan University, Grant/Award Number: ZYYC21001
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
无
最新[2023]版：
大类 | 3 区 医学
小类 | 3 区 医学：研究与实验
第一作者：
第一作者机构： [1]State Key Laboratory of Biotherapy and Cancer Center West China Hospital Sichuan University and Collaborative Innovation Center for Biotherapy Chengdu China.
共同第一作者：
通讯作者：
通讯机构： [1]State Key Laboratory of Biotherapy and Cancer Center West China Hospital Sichuan University and Collaborative Innovation Center for Biotherapy Chengdu China. [2]State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases Chinese Academy of Medical Sciences Research Unit of Oral Carcinogenesis and Management West China Hospital of Stomatology Sichuan University Chengdu China. [*1]State Key Laboratory of Biotherapy and Cancer Center,West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu 610041, China. [*2]State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Chinese Academy of Medical Sciences Research Unit of Oral Carcinogenesis and Management,West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
推荐引用方式(GB/T 7714)：
Hu Xueli,Wu Mingbo,Ma Teng,et al.Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa[J].MedComm.2022,3(4):e193.doi:10.1002/mco2.193.
APA：
Hu Xueli,Wu Mingbo,Ma Teng,Zhang Yige,Zou Chaoyu...&Zhou Xikun.(2022).Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa.MedComm,3,(4)
MLA：
Hu Xueli,et al."Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa".MedComm 3..4(2022):e193

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