机构：[1]State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, China.[2]School of Life Sciences, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, China.[3]School of Life Science and Technology, ShanghaiTech University, Shanghai 200031, China.[4]Center for Translational Medicine, Ministry of Education Key Laboratory of Birth Defects and Related Diseases of Women and Children, Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, Sichuan, China.[5]Ministry of Education Key Laboratory of Bio‑Resource and Eco‑Environment, College of Life Sciences, Sichuan University, Chengdu 610041, Sichuan, China.
CD1d-dependent type I NKT cells, which are activated by lipid antigen, are known to play important roles in innate and adaptive immunity, as are a portion of type II NKT cells. However, the heterogeneity of NKT cells, especially NKT-like cells, remains largely unknown. Here, we report the profiling of NKT (NK1.1+CD3e+) cells in livers from wild type (WT), Jα18-deficient and CD1d-deficient mice by single-cell RNA sequencing. Unbiased transcriptional clustering revealed distinct cell subsets. The transcriptomic profiles identified the well-known CD1d-dependent NKT cells and defined two CD1d-independent NKT cell subsets. In addition, validation of marker genes revealed the differential organ distribution and landscape of NKT cell subsets during liver tumor progression. More importantly, we found that CD1d-independent Sca-1-CD62L+ NKT cells showed a strong ability to secrete IFN-γ after costimulation with IL-2, IL-12 and IL-18 in vitro. Collectively, our findings provide a comprehensive characterization of NKT cell heterogeneity and unveil a previously undefined functional NKT cell subset.
基金：
National Natural Science Foundation of China (31970835, 31621003 and 31530021; Grant nos. 31771590 and
31822035), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB19000000).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
最新[2023]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
第一作者：
第一作者机构：[1]State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, China.
共同第一作者：
通讯作者：
通讯机构：[1]State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, China.[2]School of Life Sciences, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, China.[3]School of Life Science and Technology, ShanghaiTech University, Shanghai 200031, China.[4]Center for Translational Medicine, Ministry of Education Key Laboratory of Birth Defects and Related Diseases of Women and Children, Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, Sichuan, China.[5]Ministry of Education Key Laboratory of Bio‑Resource and Eco‑Environment, College of Life Sciences, Sichuan University, Chengdu 610041, Sichuan, China.
推荐引用方式(GB/T 7714)：
Shen Hao,Gu Chan,Liang Tao,et al.Unveiling the heterogeneity of NKT cells in the liver through single cell RNA sequencing.[J].Scientific reports.2020,10(1):19453.doi:10.1038/s41598-020-76659-1.
APA：
Shen Hao,Gu Chan,Liang Tao,Liu Haifeng,Guo Fan&Liu Xiaolong.(2020).Unveiling the heterogeneity of NKT cells in the liver through single cell RNA sequencing..Scientific reports,10,(1)
MLA：
Shen Hao,et al."Unveiling the heterogeneity of NKT cells in the liver through single cell RNA sequencing.".Scientific reports 10..1(2020):19453
