机构：[1]Department of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore 119615, Singapore[2]Laboratory of Human Diseases and Immunotherapies, West China Hospital, Sichuan University, 610041 Chengdu, China四川大学华西医院[3]Department of Biology, Southern University of Science and Technology, 518055 Shenzhen, China深圳市康宁医院深圳医学信息中心[4]GenEros Biopharma, 310018 Hangzhou, China[5]BGI-Shenzhen, 518033 Shenzhen, China[6]China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China[7]Department of Biotechnology and Biomedicine, Technical University of Denmark, Soltofts Plads, 2800 Kongens Lyngby, Denmark[8]School of Biology and Biological Engineering, South China University of Technology, 510006 Guangzhou, China[9]Cancer Science Institute of Singapore, YLL School of Medicine, National University of Singapore, Singapore 117599, Singapore[10]Laboratory of Translational Genomics, Division of Cancer Epidemiology & Genetics, National Cancer Institute, Gaithersburg, MD, USA[11]Endodermal Development and Differentiation Laboratory, Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138672, Singapore[12]Institute for Regenerative Medicine, University of Pennsylvania, Perelman School of Medicine, Smilow Center for Translation Research,Philadelphia, PA 19104, USA[13]State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041 Chengdu, China四川大学华西医院
The liver and gallbladder are among the most important internal organs derived from the endoderm, yet the development of the liver and gallbladder in the early embryonic stages is not fully understood. Using a transgenic Foxa2eGFP reporter mouse line, we performed single-cell full-length mRNA sequencing on endodermal and hepatic cells isolated from ten embryonic stages, ranging from E7.5 to E15.5. We identified the embryonic liver developmental trajectory from gut endoderm to hepatoblasts and characterized the transcriptome of the hepatic lineage. More importantly, we identified liver primordium as the nascent hepatic progenitors with both gut and liver features and documented dynamic gene expression during the epithelial-hepatic transition (EHT) at the stage of liver specification during E9.5-11.5. We found six groups of genes switched on or off in the EHT process, including diverse transcripitional regulators that had not been previously known to be expressed during EHT. Moreover, we identified and revealed transcriptional profiling of gallbladder primordium at E9.5. The present data provides a high-resolution resource and critical insights for understanding the liver and gallbladder development.
基金：
The work at Xin-Yuan Fu’s lab was supported by grants from the Singapore
National Medical Research Council (R-713-000-181-511), the Ministry of Education (R-
713-000-169-112), Cancer Science Institute of Singapore (CSI, Singapore) (to X.-Y.F.
grant 713001010271), the Office of Deputy President (DPRT) of National University of
Singapore, West China Hospital (Sichuan University, China) (No. 139170082), the
President Fund of SUSTech (22/Y01226237) and funds from GenEros Biopharma. The
work at BGI-Shenzhen was supported by grants from the National Natural Science
Foundation of China (No. 81672593), the Natural Science Foundation of Guangdong Province, China (No. 2018A030313379) and Science, Technology and Innovation
Commission of Shenzhen Municipality (Nos. GJHZ20180419190827179 and
KQJSCX20170322143848413).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|2 区生物学
小类|2 区生物学
最新[2023]版：
大类|1 区生物学
小类|1 区生物学
第一作者：
第一作者机构：[1]Department of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore 119615, Singapore[2]Laboratory of Human Diseases and Immunotherapies, West China Hospital, Sichuan University, 610041 Chengdu, China[3]Department of Biology, Southern University of Science and Technology, 518055 Shenzhen, China[4]GenEros Biopharma, 310018 Hangzhou, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore 119615, Singapore[2]Laboratory of Human Diseases and Immunotherapies, West China Hospital, Sichuan University, 610041 Chengdu, China[3]Department of Biology, Southern University of Science and Technology, 518055 Shenzhen, China[4]GenEros Biopharma, 310018 Hangzhou, China[5]BGI-Shenzhen, 518033 Shenzhen, China[6]China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China[9]Cancer Science Institute of Singapore, YLL School of Medicine, National University of Singapore, Singapore 117599, Singapore[13]State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041 Chengdu, China
推荐引用方式(GB/T 7714)：
Tianhao Mu,Liqin Xu,Yu Zhong,et al.Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse.[J].Communications biology.2020,3(1):642.doi:10.1038/s42003-020-01364-8.
APA：
Tianhao Mu,Liqin Xu,Yu Zhong,Xinyu Liu,Zhikun Zhao...&Yong Hou.(2020).Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse..Communications biology,3,(1)
MLA：
Tianhao Mu,et al."Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse.".Communications biology 3..1(2020):642
生物学