个人中心| | 帮助
高级检索
当前位置： 首页 > 详情页

Characterizing Stage-Specific Cellular Dynamics and Microenvironmental Remodeling in Lung Adenocarcinoma by Single-Cell RNA Sequencing

| 认领 | 导出 | |

文献详情

资源类型：
Pubmed体系：
作者：
Qing Bomiao[1,2] * ; Li Xiaolan[1,2] * ; He Xiang[1,2,3] * ; Wang Junyi[1,2,3] ; Yang Yi[4] ; Jiang Manling[1,2] ; Yan Bingbing[1,5] ; Zhang Lei[1,2,3] ; Xiong Anying[1,2] ; Ran Qin[1,2] ; Li Guoping[1,2,3] ;
机构： [1]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, 610031, China. [2]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, The Third People's Hospital of Chengdu (Affiliated Hospital of Southwest Jiaotong University), College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China. [3]Department of Pulmonary and Critical Care Medicine, Chengdu Third People's Hospital Branch of National Clinical Research Center for Respiratory Disease, Affiliated Hospital of Chongqing Medical University, Chengdu, 610031, China. [4]Department of Thoracic Surgery, Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, 610031, China. [5]Clinical Medicine Department, North Sichuan Medical College, Nanchong, 637000, China.
出处：
DOI： 10.1002/advs.202510847

关键词： hypoxia LUAD single-cell RNA sequencing TME

摘要：
Lung adenocarcinoma (LUAD) progression involves dynamic remodeling of the tumor microenvironment (TME). However, the stage-specific dynamics of immune and stromal cell remodeling throughout LUAD progression remain incompletely understood. Here, the study systematically profiles the cellular composition and transcriptional states across multiple LUAD stages, integrating early-stage patient specimens with publicly available datasets encompassing advanced-stage disease. The analysis reveals a marked stage-dependent shift from a proliferative and immune-activated microenvironment in early LUAD to a hypoxia-enriched and immunosuppressive landscape in advanced disease. A distinct hypoxia-adapted epithelial tumor cell subpopulation (C5), exhibiting transcriptional features of metastasis, invasion, and hypoxia, and poor prognosis, is identified. Advanced LUAD featured immunosuppressive LGMN⁺ macrophages and STAT1-driven exhausted CD8⁺ T cells. FKBP11⁺ plasma B cells exhibited exhaustion-linked metabolic changes. POSTN⁺ CAFs emerged as central mediators of extracellular matrix (ECM) remodeling and immune exclusion. Collectively, the findings reveal a model of hypoxia-driven functional convergence, in which distinct TME components co-evolve toward phenotypes that collectively promote immune evasion, matrix remodeling, and tumor progression. These findings may provide insights into stage-specific cellular dynamics and highlight promising therapeutic targets for precision immunotherapy strategies.© 2025 The Author(s). Advanced Science published by Wiley‐VCH GmbH.

基金：
This work was supported by the National Natural Science Foundation of China (82370022, 82370023, and 82300088); Natural Science Foundation of Sichuan Province (23NSFSC1736, 2024NSFSC1639), The Third People’s Hospital of Chengdu Clinical Research Program (CSY-YN-01-2023- 002, CSY-YN-03-2024-027, and CSY-YN-01-2023-048), The Third People’s Hospital of Chengdu Scientific Research Project (2023PI01), and Chengdu High-level Key Clinical Specialty Construction Project (ZX20201202020).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2025]版：
大类 | 1 区 综合性期刊
小类 | 1 区 化学：综合 1 区 材料科学：综合 1 区 纳米科技
最新[2025]版：
大类 | 1 区 综合性期刊
小类 | 1 区 化学：综合 1 区 材料科学：综合 1 区 纳米科技
第一作者：
第一作者机构： [1]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, 610031, China. [2]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, The Third People's Hospital of Chengdu (Affiliated Hospital of Southwest Jiaotong University), College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China.
共同第一作者：
通讯作者：
通讯机构： [1]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, 610031, China. [2]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, The Third People's Hospital of Chengdu (Affiliated Hospital of Southwest Jiaotong University), College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China. [3]Department of Pulmonary and Critical Care Medicine, Chengdu Third People's Hospital Branch of National Clinical Research Center for Respiratory Disease, Affiliated Hospital of Chongqing Medical University, Chengdu, 610031, China.
推荐引用方式(GB/T 7714)：
Qing Bomiao,Li Xiaolan,He Xiang,et al.Characterizing Stage-Specific Cellular Dynamics and Microenvironmental Remodeling in Lung Adenocarcinoma by Single-Cell RNA Sequencing[J].Advanced Science (Weinheim, Baden-Wurttemberg, Germany).2025,e10847.doi:10.1002/advs.202510847.
APA：
Qing Bomiao,Li Xiaolan,He Xiang,Wang Junyi,Yang Yi...&Li Guoping.(2025).Characterizing Stage-Specific Cellular Dynamics and Microenvironmental Remodeling in Lung Adenocarcinoma by Single-Cell RNA Sequencing.Advanced Science (Weinheim, Baden-Wurttemberg, Germany),,
MLA：
Qing Bomiao,et al."Characterizing Stage-Specific Cellular Dynamics and Microenvironmental Remodeling in Lung Adenocarcinoma by Single-Cell RNA Sequencing".Advanced Science (Weinheim, Baden-Wurttemberg, Germany) .(2025):e10847

相关文献

[1]Unveiling the heterogeneity of NKT cells in the liver through single cell RNA sequencing. [2]Single-Cell RNA Sequencing Reveals the Pathogenic Relevance of Intracranial Atherosclerosis in Blood Blister-Like Aneurysms [3]Single-cell RNA sequencing revealed PPARG promoted osteosarcoma progression: based on osteoclast proliferation [4]Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse. [5]Single-cell RNA sequencing uncovers a neuron-like macrophage subset associated with cancer pain [6]Single-cell RNA sequencing highlights intratumor heterogeneity and intercellular network featured in adamantinomatous craniopharyngioma [7]Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing-Assisted Multi-Omics Analysis. [8]Unveiling the NEFH+ malignant cell subtype: Insights from single-cell RNA sequencing in prostate cancer progression and tumor microenvironment interactions [9]Single-cell RNA sequencing reveals classical monocytes are the major precursors of rat osteoclasts [10]Single-cell RNA Sequencing Analysis Reveals Cancer-associated Fibroblast Signature for Prediction of Clinical Outcomes and Immunotherapy in Gastric Cancer

资源点击量：65768 今日访问量：2 总访问量：5150 更新日期：2025-12-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 四川省肿瘤医院 技术支持：重庆聚合科技有限公司 地址：成都市人民南路四段55号