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Prediction of Potential Biomarkers in Early-Stage Nasopharyngeal Carcinoma Based on Platelet RNA Sequencing

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机构: [1]Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, No.420, Fuma Road, Fuzhou, 350014, Fujian, China. [2]Cancer Center, West China Xiamen Hospital of Sichuan University, Xiamen, Fujian, China. [3]Institute of Respiratory Diseases, Xiamen Medical College, Xiamen, Fujian, China. [4]Fujian Collaborative Innovation Center for Accurate Medicine of Respiratory Diseases, Xiamen, Fujian, China. [5]Xiamen LifeInt Technology Co., Ltd, Xiamen, Fujian, China. [6]Innovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian, China.
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关键词: Nasopharyngeal carcinoma Tumour-educated blood platelets Differentially expressed mRNAs Differentially expressed lncRNAs Biomarkers

摘要:
Early diagnosis is essential for the treatment and prevention of nasopharyngeal cancer. However, there is a lack of effective biological indicators for nasopharyngeal carcinoma (NPC). Therefore, we explored the potential biomarkers in tumour-educated blood platelet (TEP) RNA in early NPC. Platelets were isolated from blood plasma and their RNA was extracted. High-throughput sequenced data from a total of 33 plasma samples were analysed using DESeq2 to identify the differentially expressed genes (DEGs). Subsequently, the DEGs were subjected to principal component analysis (PCA), gene ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis; and Cytoscape, TargetScan, and miRanda software were used for inferring the competing endogenous RNA network. We identified 19 long non-coding (lnc) RNAs (DElncRNAs) and 248 mRNAs (DEmRNAs) that were differentially expressed in the TEP RNA. In addition, SELP gene mRNA and lncRNAs AC092135.3, AC012358.2, AL021807.1, AP001972.5, and GPX1 were found to be down-regulated DEmRNA and DElncRNAs in the early stage of NPC. Bioinformatic analysis showed that these DEmRNAs and DElncRNAs may be involved in regulating the pathogenesis of NPC. Our research may provide new insights for exploring the biological mechanisms of NPC and early diagnosis using potential biomarkers.© 2022. The Author(s).

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出版当年[2022]版:
大类 | 4 区 医学
小类 | 4 区 生化与分子生物学 4 区 生物工程与应用微生物
最新[2023]版:
大类 | 4 区 生物学
小类 | 4 区 生化与分子生物学 4 区 生物工程与应用微生物
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第一作者机构: [1]Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, No.420, Fuma Road, Fuzhou, 350014, Fujian, China.
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通讯机构: [1]Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, No.420, Fuma Road, Fuzhou, 350014, Fujian, China. [6]Innovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian, China.
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