A novel fluorescent aptasensor for ultrasensitive detection of Helicobacter pylori in stool samples based on catalytic hairpin assembly cascade hybridization chain reaction
机构：[1]West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China[2]Department of Clinical Laboratory, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China四川省人民医院四川省肿瘤医院
In this work, a fluorescent aptasensor based on enzyme-free catalytic hairpin assembly cascade hybridization chain reaction (CHA-HCR) was established for rapid and sensitive detection of Helicobacter pylori (H. pylori) in stool samples. Four single-stranded DNA hairpins (HP1, HP2, HP3 and HP4), an aptamer and one trigger strand (T strand) were designed, and the stem of HP3 hairpin was modified with a fluorophore (Cy3) and a quencher (BHQ2), respectively. When H. pylori specifically bonded to the aptamer, the T strand was released from aptamer strand. Consequently, the free T strand triggered the CHA-HCR system, and HP2-HP1-[HP3-HP4]n long double strand complex was produced, resulting in the significant fluorescent enhancement. The proposed aptasensor can detect H. pylori in a wide concentration range (20 cfu/mL~ 1000 cfu/mL) with the detection limit as low as 5 cfu/mL. What's more, the fluorescent aptasensor was implemented to detect H. pylori in 102 stool samples and the results were highly consistent with those of 13C urea breath test (13C-UBT), which demonstrated that the fluorescent aptasensor possessed enormous potential to rapid screening of H. pylori among the whole population and showed novel perspectives in the analysis of other biomarkers, clinical diagnostics, and biomedical applications.
基金：
We acknowledge the support funded by China Postdoctoral Science Foundations (Grant No.: 2019M663531, and BX20190221), and Chengdu Municipal Bureau on Science and Technology (Grant No.: 2021-YF05-00592-SN), and Key Research and Development Program of Sichuan Province (Grant No.: 2022ZYF1927 and 2022YFS0513).
第一作者机构：[2]Department of Clinical Laboratory, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China
通讯作者：
通讯机构：[1]West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China[*1]West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
推荐引用方式(GB/T 7714)：
Zou Haimin,Lin Chiliang,Zan Haocheng,et al.A novel fluorescent aptasensor for ultrasensitive detection of Helicobacter pylori in stool samples based on catalytic hairpin assembly cascade hybridization chain reaction[J].SENSORS AND ACTUATORS B-CHEMICAL.2022,368:doi:10.1016/j.snb.2022.132157.
APA：
Zou, Haimin,Lin, Chiliang,Zan, Haocheng,Hu, Yuyao,Xu, Xiaruiyan...&Zhou, Chen.(2022).A novel fluorescent aptasensor for ultrasensitive detection of Helicobacter pylori in stool samples based on catalytic hairpin assembly cascade hybridization chain reaction.SENSORS AND ACTUATORS B-CHEMICAL,368,
MLA：
Zou, Haimin,et al."A novel fluorescent aptasensor for ultrasensitive detection of Helicobacter pylori in stool samples based on catalytic hairpin assembly cascade hybridization chain reaction".SENSORS AND ACTUATORS B-CHEMICAL 368.(2022)
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